Hotspot

rps18

Genome · 133514-133987 · 474 bp

Back to Geranium

Pi 0.0566

MarkerSeek score 75.00

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: Genome
length_bp: 474
variable_sites: 26
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TCTGCCCCCATTGTTTCAAA	CGTTTGTGGATCACTCGGAT	45.0	50.0	57.9	58.0	1305-1314 bp	1.0	83.3
rps18_p2	TTCTGCCCCCATTGTTTCAA	CGTTTGTGGATCACTCGGAT	45.0	50.0	57.9	58.0	1306-1315 bp	1.0	83.3
rps18_p3	TCTGCCCCCATTGTTTCAAA	CTCGAGGGGCTTATTCAACC	45.0	55.0	57.9	58.0	1409-1418 bp	1.0	82.8
rps18_p4	TCTGCCCCCATTGTTTCAAA	GAGGATGCACTCAATCAGGG	45.0	55.0	57.9	58.0	952 bp	0.5	59.0
rps18_p5	TTCTGCCCCCATTGTTTCAA	GAGGATGCACTCAATCAGGG	45.0	55.0	57.9	58.0	953 bp	0.5	59.0