Hotspot

rps15

Genome · 44625-44909 · 285 bp

Back to Geranium

Pi 0.0573

MarkerSeek score 77.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 285
variable_sites: 16
pi: 0.0573
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	ACGAAACTAAATTTATCCAAATGCA	AAAGATGGGTAGGAGGCCC	28.0	57.9	56.3	58.4	421-427 bp	1.0	44.2
rps15_p2	ACGAAACTAAATTTATCCAAATGCA	AAAAAGATGGGTAGGAGGCC	28.0	50.0	56.3	57.2	423-429 bp	1.0	44.1
rps15_p3	ACGAAACTAAATTTATCCAAATGCA	AAAAGATGGGTAGGAGGCCC	28.0	55.0	56.3	59.1	422-428 bp	1.0	44.1
rps15_p4	AACGAAACTAAATTTATCCAAATGCA	AAAAAGATGGGTAGGAGGCC	26.9	50.0	57.0	57.2	424-430 bp	1.0	44.1
rps15_p5	ACGAAACTAAATTTATCCAAATGCAA	AAAAAGATGGGTAGGAGGCC	26.9	50.0	57.0	57.2	423-429 bp	1.0	44.1