Hotspot

rps12-trnI

Genome · 135627-137159 · 1533 bp

Back to Geranium

Pi 0.0824

MarkerSeek score 82.40

Primer available yes

Label rps12-trnI

Feature Metadata

label_name: rps12-trnI
feature_type: igs
region: Genome
length_bp: 1533
variable_sites: 124
pi: 0.0824
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnI_p1	TGGCATTTCTGATTGGCTGT	GTTGGGCGCTTTAACCATTC	45.0	50.0	58.1	58.0	1598-1620 bp	1.0	86.9
rps12-trnI_p2	GTGGCATTTCTGATTGGCTG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.7	58.0	1599-1621 bp	1.0	85.2
rps12-trnI_p3	TGGCATTTCTGATTGGCTGT	AGTTGGGCGCTTTAACCATT	45.0	45.0	58.1	58.4	1599-1621 bp	1.0	84.0
rps12-trnI_p4	TGGCATTTCTGATTGGCTGT	TTGGGCGCTTTAACCATTCA	45.0	45.0	58.1	58.4	1597-1619 bp	1.0	84.0
rps12-trnI_p5	TGGCATTTCTGATTGGCTGT	TGAACCTACGAATTCGCCAA	45.0	45.0	58.1	57.5	1624-1646 bp	1.0	83.2