Hotspot

ndhC-trnM

Genome · 111230-115323 · 4094 bp

Back to Geranium

Pi 0.0755

MarkerSeek score 86.30

Primer available yes

Label ndhC-trnM

Feature Metadata

label_name: ndhC-trnM
feature_type: igs
region: Genome
length_bp: 4094
variable_sites: 289
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM_p1	GGGAGTGCTCAAGTTCCAAA	CAAAAACGCGAGGGAACATC	50.0	50.0	58.0	58.0	709-767 bp	1.0	91.6
ndhC-trnM_p2	TCCTACGTATCAGTCGGCAT	CAAAAACGCGAGGGAACATC	50.0	50.0	58.0	58.0	863-921 bp	1.0	91.5
ndhC-trnM_p3	CGGAAGCCCCCATTTTCATA	CAAAAACGCGAGGGAACATC	50.0	50.0	57.9	58.0	824-882 bp	1.0	91.2
ndhC-trnM_p4	GTCCGAATCCGTTCTGTTGA	CAAAAACGCGAGGGAACATC	50.0	50.0	57.9	58.0	672-730 bp	1.0	91.1
ndhC-trnM_p5	ACTCTATCTCCGCGTGCTAT	CAAAAACGCGAGGGAACATC	50.0	50.0	58.1	58.0	594-658 bp	1.0	90.9