Hotspot

trnE-rps11

Genome · 86911-88532 · 1622 bp

Back to Geranium

Pi 0.0581

MarkerSeek score 77.20

Primer available yes

Label trnE-rps11

Feature Metadata

label_name: trnE-rps11
feature_type: igs
region: Genome
length_bp: 1622
variable_sites: 91
pi: 0.0581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-rps11_p1	GAAGTGAGAAGTGAGAGCGG	CACACGCGTTATCGATTTCG	55.0	50.0	58.0	58.0	2359 bp	0.5	59.7
trnE-rps11_p2	GAAGTGAGAAGTGAGAGCGG	GACCCGTAAACTCCCCATTC	55.0	55.0	58.0	58.0	2271 bp	0.5	59.6
trnE-rps11_p3	GAAGTGAGAAGTGAGAGCGG	AAGATTTCCACCAGCCCAAG	55.0	50.0	58.0	58.1	2527 bp	0.5	59.3
trnE-rps11_p4	GAAGTGAGAAGTGAGAGCGG	GAAAGATTTCCACCAGCCCA	55.0	50.0	58.0	58.1	2529 bp	0.5	59.3
trnE-rps11_p5	GAAGTGAGAAGTGAGAGCGG	GCAGGCTTTGTAACTCCCTT	55.0	50.0	58.0	58.1	2461 bp	0.5	59.2