Hotspot

trnQ-rpl23

Genome · 65518-68913 · 3396 bp

Back to Geranium

Pi 0.0815

MarkerSeek score 74.70

Primer available yes

Label trnQ-rpl23

Feature Metadata

label_name: trnQ-rpl23
feature_type: igs
region: Genome
length_bp: 3396
variable_sites: 274
pi: 0.0815
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-rpl23_p1	CGGGTTTTCTGATGACGCTA	CGGTACTAATTTGGGGGCTC	50.0	55.0	58.0	58.0	944 bp	0.5	59.7
trnQ-rpl23_p2	GCGAACATCAATTGCAACGA	CGGTACTAATTTGGGGGCTC	45.0	55.0	58.0	58.0	363 bp	0.5	59.4
trnQ-rpl23_p3	GCGAACATCAATTGCAACGA	GAAAGGGAAAGGGGTCGATC	45.0	55.0	58.0	58.0	485 bp	0.5	59.4
trnQ-rpl23_p4	TTCCAACTAAGCGTTCTCGG	CGGTACTAATTTGGGGGCTC	50.0	55.0	57.9	58.0	585 bp	0.5	59.1
trnQ-rpl23_p5	TTCCAACTAAGCGTTCTCGG	GAAAGGGAAAGGGGTCGATC	50.0	55.0	57.9	58.0	707 bp	0.5	59.1