Hotspot

rpl23-rps12

Genome · 24714-25576 · 863 bp

Back to Geranium

Pi 0.136

MarkerSeek score 83.50

Primer available yes

Label rpl23-rps12

Feature Metadata

label_name: rpl23-rps12
feature_type: igs
region: Genome
length_bp: 863
variable_sites: 109
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rps12_p1	ATGGGACCGGACCTAGTATG	CGAATACGGCTTTCCACAGA	55.0	50.0	58.0	58.0	1620 bp	0.5	59.9
rpl23-rps12_p2	ATGGGACCGGACCTAGTATG	TCGAATACGGCTTTCCACAG	55.0	50.0	58.0	58.0	1621 bp	0.5	59.9
rpl23-rps12_p3	ATGGGACCGGACCTAGTATG	TACTCCGACAGCATCTAGGG	55.0	55.0	58.0	58.0	1118 bp	0.5	59.8
rpl23-rps12_p4	ATGGGACCGGACCTAGTATG	TCTACAACGCACTAGAACGC	55.0	50.0	58.0	58.0	1155 bp	0.5	59.7
rpl23-rps12_p5	AAAGAATGGGACCGGACCTA	CGAATACGGCTTTCCACAGA	50.0	50.0	58.0	58.0	1625 bp	0.5	59.7