Hotspot

psbA-psbK

Genome · 1484-5299 · 3816 bp

Back to Geranium

Pi 0.0533

MarkerSeek score 78.00

Primer available yes

Label psbA-psbK

Feature Metadata

label_name: psbA-psbK
feature_type: igs
region: Genome
length_bp: 3816
variable_sites: 201
pi: 0.0533
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-psbK_p1	TTTTTCCACCCTTAGCACCC	GAAGGCGGGAATTTTTGCTC	50.0	50.0	58.0	58.0	572 bp	0.5	59.9
psbA-psbK_p2	TGAATGACCCGCTTAACTCG	GAAGGCGGGAATTTTTGCTC	50.0	50.0	58.0	58.0	833 bp	0.5	59.9
psbA-psbK_p3	TTTTTCCACCCTTAGCACCC	GGGGTGCACTATTTTTGGGA	50.0	50.0	58.0	58.1	830 bp	0.5	59.4
psbA-psbK_p4	TGAATGACCCGCTTAACTCG	GGGGTGCACTATTTTTGGGA	50.0	50.0	58.0	58.1	1091 bp	0.5	59.4
psbA-psbK_p5	GCCTTTGGCGTCGTATCTTA	GAAGGCGGGAATTTTTGCTC	50.0	50.0	58.1	58.0	356 bp	0.5	59.4