primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbA-psbK_p1 psbA-psbK 1 TTTTTCCACCCTTAGCACCC GAAGGCGGGAATTTTTGCTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.007645 4017 4136 572-572 0.500000 59.9 psbA-psbK_p2 psbA-psbK 2 TGAATGACCCGCTTAACTCG GAAGGCGGGAATTTTTGCTC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.008291 4017 4136 833-833 0.500000 59.9 psbA-psbK_p3 psbA-psbK 3 TTTTTCCACCCTTAGCACCC GGGGTGCACTATTTTTGGGA 20 20 50.0 50.0 58.0 58.1 0.0 18.0 0.072302 4017 4136 830-830 0.500000 59.4 psbA-psbK_p4 psbA-psbK 4 TGAATGACCCGCTTAACTCG GGGGTGCACTATTTTTGGGA 20 20 50.0 50.0 58.0 58.1 0.0 18.0 0.072947 4017 4136 1091-1091 0.500000 59.4 psbA-psbK_p5 psbA-psbK 5 GCCTTTGGCGTCGTATCTTA GAAGGCGGGAATTTTTGCTC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.073141 4017 4136 356-356 0.500000 59.4 trnI-rpl16_p1 trnI-rpl16 1 GTTGGGCGCTTTAACCATTC AATACGACTGCCTTGAGAGG 20 20 50.0 50.0 58.0 57.0 7.6 0.0 0.978829 12878 13610 844-846 1.000000 77.3 trnI-rpl16_p2 trnI-rpl16 2 GTTGGGCGCTTTAACCATTC CCGAAAGAAATACGACTGCC 20 20 50.0 50.0 58.0 56.9 7.6 0.0 1.096611 12878 13610 852-854 1.000000 76.3 trnI-rpl16_p3 trnI-rpl16 3 GTTGGGCGCTTTAACCATTC AAATACGACTGCCTTGAGAGG 20 21 50.0 47.6 58.0 57.7 7.6 0.0 1.262175 12878 13610 845-847 1.000000 75.0 trnI-rpl16_p4 trnI-rpl16 4 AGTTGGGCGCTTTAACCATT AATACGACTGCCTTGAGAGG 20 20 45.0 50.0 58.4 57.0 7.6 0.0 1.341764 12878 13610 845-847 1.000000 74.4 trnI-rpl16_p5 trnI-rpl16 5 TTGGGCGCTTTAACCATTCA AATACGACTGCCTTGAGAGG 20 20 45.0 50.0 58.4 57.0 7.6 0.0 1.347636 12878 13610 843-845 1.000000 74.3 rpl14-rps8_p1 rpl14-rps8 1 AATTTCCCTAGCGCCTGAAG AACCGTCTCTTTGTTAGCCA 20 20 50.0 45.0 58.0 57.1 2.3 0.0 0.966862 14542 14628 185-187 1.000000 75.7 rpl14-rps8_p2 rpl14-rps8 2 GTGCAATCACCCACGAATTG AACCGTCTCTTTGTTAGCCA 20 20 50.0 45.0 58.0 57.1 0.0 0.0 0.941486 14542 14628 228-230 1.000000 75.3 rpl14-rps8_p3 rpl14-rps8 3 TGCAATCACCCACGAATTGA AACCGTCTCTTTGTTAGCCA 20 20 45.0 45.0 58.1 57.1 0.0 0.0 1.019802 14542 14628 227-229 1.000000 74.6 rpl14-rps8_p4 rpl14-rps8 4 AATCCAAAAGGAACTCGCGT AACCGTCTCTTTGTTAGCCA 20 20 45.0 45.0 58.1 57.1 5.8 0.0 1.030530 14542 14628 253-255 1.000000 74.3 rpl14-rps8_p5 rpl14-rps8 5 ATCCAAAAGGAACTCGCGTT AACCGTCTCTTTGTTAGCCA 20 20 45.0 45.0 58.1 57.1 5.8 0.0 1.030530 14542 14628 252-254 1.000000 74.3 rps11-rpoA_p1 rps11-rpoA 1 AACCCCTATGCCACATAACG GACTCTTACTTTTTCTCGAATCA 20 23 50.0 34.8 57.7 54.5 0.0 0.0 6.878525 16212 16281 140-146 1.000000 46.2 rps11-rpoA_p2 rps11-rpoA 2 AACCCCTATGCCACATAACG ACTCTTACTTTTTCTCGAATCA 20 22 50.0 31.8 57.7 53.2 0.0 0.0 7.158537 16212 16281 139-145 1.000000 46.2 rps11-rpoA_p3 rps11-rpoA 3 AACCCCTATGCCACATAACG GACTCTTACTTTTTCTCGAATCAT 20 24 50.0 33.3 57.7 55.0 0.0 0.0 7.342776 16212 16281 140-146 1.000000 46.2 rps11-rpoA_p4 rps11-rpoA 4 AACCCCTATGCCACATAACG ACTCTTACTTTTTCTCGAATCAT 20 23 50.0 30.4 57.7 53.8 0.0 0.0 7.541455 16212 16281 139-145 1.000000 46.2 rps11-rpoA_p5 rps11-rpoA 5 CGGGATATAACCCCTATGCC GACTCTTACTTTTTCTCGAATCA 20 23 55.0 34.8 57.0 54.5 12.3 0.0 7.539135 16212 16281 148-154 1.000000 45.8 rpl23-rps12_p1 rpl23-rps12 1 ATGGGACCGGACCTAGTATG CGAATACGGCTTTCCACAGA 20 20 55.0 50.0 58.0 58.0 22.4 0.0 0.015705 24714 25576 1620-1620 0.500000 59.9 rpl23-rps12_p2 rpl23-rps12 2 ATGGGACCGGACCTAGTATG TCGAATACGGCTTTCCACAG 20 20 55.0 50.0 58.0 58.0 22.4 0.0 0.015705 24714 25576 1621-1621 0.500000 59.9 rpl23-rps12_p3 rpl23-rps12 3 ATGGGACCGGACCTAGTATG TACTCCGACAGCATCTAGGG 20 20 55.0 55.0 58.0 58.0 22.4 0.0 0.028462 24714 25576 1118-1118 0.500000 59.8 rpl23-rps12_p4 rpl23-rps12 4 ATGGGACCGGACCTAGTATG TCTACAACGCACTAGAACGC 20 20 55.0 50.0 58.0 58.0 22.4 0.0 0.037984 24714 25576 1155-1155 0.500000 59.7 rpl23-rps12_p5 rpl23-rps12 5 AAAGAATGGGACCGGACCTA CGAATACGGCTTTCCACAGA 20 20 50.0 50.0 58.0 58.0 22.4 0.0 0.043450 24714 25576 1625-1625 0.500000 59.7 rps15_p1 rps15 1 ACGAAACTAAATTTATCCAAATGCA AAAGATGGGTAGGAGGCCC 25 19 28.0 57.9 56.3 58.4 0.0 0.0 8.027636 44625 44909 421-427 1.000000 44.2 rps15_p2 rps15 2 ACGAAACTAAATTTATCCAAATGCA AAAAAGATGGGTAGGAGGCC 25 20 28.0 50.0 56.3 57.2 0.0 0.0 7.490397 44625 44909 423-429 1.000000 44.1 rps15_p3 rps15 3 ACGAAACTAAATTTATCCAAATGCA AAAAGATGGGTAGGAGGCCC 25 20 28.0 55.0 56.3 59.1 0.0 0.0 7.734707 44625 44909 422-428 1.000000 44.1 rps15_p4 rps15 4 AACGAAACTAAATTTATCCAAATGCA AAAAAGATGGGTAGGAGGCC 26 20 26.9 50.0 57.0 57.2 0.0 0.0 7.878300 44625 44909 424-430 1.000000 44.1 rps15_p5 rps15 5 ACGAAACTAAATTTATCCAAATGCAA AAAAAGATGGGTAGGAGGCC 26 20 26.9 50.0 57.0 57.2 0.0 0.0 7.878300 44625 44909 423-429 1.000000 44.1 trnI-rrn16S_p1 trnI-rrn16S 1 CTATCCATGTGGCTGAGAGC GAAGGTGTGGCTGGATTACC 20 20 55.0 55.0 57.8 58.2 0.0 0.0 0.415384 53914 56534 417-2731 1.000000 86.4 trnI-rrn16S_p2 trnI-rrn16S 2 CTATCCATGTGGCTGAGAGC GGAAGGTGTGGCTGGATTAC 20 20 55.0 55.0 57.8 58.2 0.0 0.0 0.415384 53914 56534 418-2732 1.000000 86.4 trnI-rrn16S_p3 trnI-rrn16S 3 CTATCCATGTGGCTGAGAGC CAAGGTAGCCGTACTGGAAG 20 20 55.0 55.0 57.8 57.7 0.0 7.8 0.462074 53914 56534 433-2747 1.000000 86.0 trnI-rrn16S_p4 trnI-rrn16S 4 GGCACAACGACGCAATTATC GAAGGTGTGGCTGGATTACC 20 20 50.0 55.0 58.2 58.2 0.0 0.0 0.438366 53914 56534 2699-2990 1.000000 82.3 trnI-rrn16S_p5 trnI-rrn16S 5 GGCACAACGACGCAATTATC GGAAGGTGTGGCTGGATTAC 20 20 50.0 55.0 58.2 58.2 0.0 0.0 0.438366 53914 56534 2700-2991 1.000000 82.3 rrn16S-trnV_p1 rrn16S-trnV 1 TTTCCAGCTGTTGTTCCCC GGGGCAGGGAAGGGATATAA 19 20 52.6 55.0 57.9 58.2 13.8 0.0 1.370321 58011 58822 930-1102 1.000000 76.0 rrn16S-trnV_p2 rrn16S-trnV 2 TTTCCAGCTGTTGTTCCCC GACGAGGTGGAAGTCATCAG 19 20 52.6 55.0 57.9 57.7 13.8 0.0 1.411347 58011 58822 888-1060 1.000000 75.8 rrn16S-trnV_p3 rrn16S-trnV 3 TTTCCAGCTGTTGTTCCCC GGAAGTCATCAGTTCGAGCC 19 20 52.6 55.0 57.9 58.4 13.8 0.0 1.479331 58011 58822 880-1052 1.000000 75.3 rrn16S-trnV_p4 rrn16S-trnV 4 TTTCCAGCTGTTGTTCCCC ACGAGGTGGAAGTCATCAGT 19 20 52.6 50.0 57.9 58.4 13.8 0.0 1.491747 58011 58822 887-1059 1.000000 75.2 rrn16S-trnV_p5 rrn16S-trnV 5 TTTCCAGCTGTTGTTCCCC TGGAAGTCATCAGTTCGAGC 19 20 52.6 50.0 57.9 57.6 13.8 0.0 1.495801 58011 58822 881-1053 1.000000 75.2 rpl23_p1 rpl23 1 CCACAACGATGCTGTCCATA CGACACCAGACAATACCCAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.032910 64418 65967 743-2276 1.000000 88.8 rpl23_p2 rpl23 2 CGCCTCTGACCATCAAATGA CGACACCAGACAATACCCAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.038565 64418 65967 1333-2866 1.000000 87.4 rpl23_p3 rpl23 3 CAATTCGCCTCTGACCATCA CGACACCAGACAATACCCAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.038565 64418 65967 1338-2871 1.000000 87.3 rpl23_p4 rpl23 4 TCGCCTCTGACCATCAAATG CGACACCAGACAATACCCAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.038565 64418 65967 1334-2867 1.000000 87.3 rpl23_p5 rpl23 5 CCACAACGATGCTGTCCATA GGGGATTCATGGCGAAGATT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.035882 64418 65967 2359-2359 0.500000 59.7 trnQ-rpl23_p1 trnQ-rpl23 1 CGGGTTTTCTGATGACGCTA CGGTACTAATTTGGGGGCTC 20 20 50.0 55.0 58.0 58.0 6.5 0.0 0.041411 68513 68632 944-944 0.500000 59.7 trnQ-rpl23_p2 trnQ-rpl23 2 GCGAACATCAATTGCAACGA CGGTACTAATTTGGGGGCTC 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.074416 68513 68632 363-363 0.500000 59.4 trnQ-rpl23_p3 trnQ-rpl23 3 GCGAACATCAATTGCAACGA GAAAGGGAAAGGGGTCGATC 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.077406 68513 68632 485-485 0.500000 59.4 trnQ-rpl23_p4 trnQ-rpl23 4 TTCCAACTAAGCGTTCTCGG CGGTACTAATTTGGGGGCTC 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.111375 68513 68632 585-585 0.500000 59.1 trnQ-rpl23_p5 trnQ-rpl23 5 TTCCAACTAAGCGTTCTCGG GAAAGGGAAAGGGGTCGATC 20 20 50.0 55.0 57.9 58.0 0.0 0.0 0.114365 68513 68632 707-707 0.500000 59.1 trnE-rps11_p1 trnE-rps11 1 GAAGTGAGAAGTGAGAGCGG CACACGCGTTATCGATTTCG 20 20 55.0 50.0 58.0 58.0 0.0 10.2 0.037182 86911 88532 2359-2359 0.500000 59.7 trnE-rps11_p2 trnE-rps11 2 GAAGTGAGAAGTGAGAGCGG GACCCGTAAACTCCCCATTC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.044674 86911 88532 2271-2271 0.500000 59.6 trnE-rps11_p3 trnE-rps11 3 GAAGTGAGAAGTGAGAGCGG AAGATTTCCACCAGCCCAAG 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.081701 86911 88532 2527-2527 0.500000 59.3 trnE-rps11_p4 trnE-rps11 4 GAAGTGAGAAGTGAGAGCGG GAAAGATTTCCACCAGCCCA 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.087214 86911 88532 2529-2529 0.500000 59.3 trnE-rps11_p5 trnE-rps11 5 GAAGTGAGAAGTGAGAGCGG GCAGGCTTTGTAACTCCCTT 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.099512 86911 88532 2461-2461 0.500000 59.2 rps11_p1 rps11 1 CAGTTTCGTTTTAACATGAGATGGA AAGCAGGCTTTGTAACTCCC 25 20 36.0 50.0 58.1 58.1 0.0 0.0 5.190901 88533 88814 480-494 1.000000 48.3 rps11_p2 rps11 2 CAGTTTCGTTTTAACATGAGATGGA GCAGGCTTTGTAACTCCCTT 25 20 36.0 50.0 58.1 58.1 0.0 0.0 5.190901 88533 88814 478-492 1.000000 48.3 rps11_p3 rps11 3 TCAGTTTCGTTTTAACATGAGATGG AAGCAGGCTTTGTAACTCCC 25 20 36.0 50.0 58.1 58.1 0.0 0.0 5.190901 88533 88814 481-495 1.000000 48.3 rps11_p4 rps11 4 TCAGTTTCGTTTTAACATGAGATGG GCAGGCTTTGTAACTCCCTT 25 20 36.0 50.0 58.1 58.1 0.0 0.0 5.190901 88533 88814 479-493 1.000000 48.3 rps11_p5 rps11 5 AGTTTCGTTTTAACATGAGATGGA GCAGGCTTTGTAACTCCCTT 24 20 33.3 50.0 56.9 58.1 0.0 0.0 5.209310 88533 88814 477-491 1.000000 48.3 ndhC-trnM_p1 ndhC-trnM 1 GGGAGTGCTCAAGTTCCAAA CAAAAACGCGAGGGAACATC 20 20 50.0 50.0 58.0 58.0 1.4 15.9 0.054997 113105 113224 709-767 1.000000 91.6 ndhC-trnM_p2 ndhC-trnM 2 TCCTACGTATCAGTCGGCAT CAAAAACGCGAGGGAACATC 20 20 50.0 50.0 58.0 58.0 0.0 15.9 0.059197 113105 113224 863-921 1.000000 91.5 ndhC-trnM_p3 ndhC-trnM 3 CGGAAGCCCCCATTTTCATA CAAAAACGCGAGGGAACATC 20 20 50.0 50.0 57.9 58.0 0.0 15.9 0.102152 113105 113224 824-882 1.000000 91.2 ndhC-trnM_p4 ndhC-trnM 4 GTCCGAATCCGTTCTGTTGA CAAAAACGCGAGGGAACATC 20 20 50.0 50.0 57.9 58.0 0.0 15.9 0.110800 113105 113224 672-730 1.000000 91.1 ndhC-trnM_p5 ndhC-trnM 5 ACTCTATCTCCGCGTGCTAT CAAAAACGCGAGGGAACATC 20 20 50.0 50.0 58.1 58.0 4.9 15.9 0.137351 113105 113224 594-658 1.000000 90.9 petA-psbJ_p1 petA-psbJ 1 TGTGCTTCTTGGCATCTGTT GCTGGTATTCCTGTGATCGG 20 20 45.0 55.0 58.0 58.1 0.0 0.0 0.143447 128100 129345 1390-1416 1.000000 82.0 petA-psbJ_p2 petA-psbJ 2 TTGTGCTTCTTGGCATCTGT GCTGGTATTCCTGTGATCGG 20 20 45.0 55.0 58.0 58.1 0.0 0.0 0.143447 128100 129345 1391-1417 1.000000 82.0 petA-psbJ_p3 petA-psbJ 3 TCAAGGCCTTTTGTGCTTCT GCTGGTATTCCTGTGATCGG 20 20 45.0 55.0 57.9 58.1 17.6 0.0 0.200697 128100 129345 1401-1427 1.000000 81.6 petA-psbJ_p4 petA-psbJ 4 CGTGTTCAAGGCCTTTTGTG GCTGGTATTCCTGTGATCGG 20 20 50.0 55.0 58.2 58.1 17.6 0.0 0.290007 128100 129345 1406-1432 1.000000 80.8 petA-psbJ_p5 petA-psbJ 5 TGTGCTTCTTGGCATCTGTT ATACTCCGGGTTGGGTTCAT 20 20 45.0 50.0 58.0 58.4 0.0 0.0 0.422758 128100 129345 1343-1369 1.000000 79.9 rps18_p1 rps18 1 TCTGCCCCCATTGTTTCAAA CGTTTGTGGATCACTCGGAT 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.104245 133514 133987 1305-1314 1.000000 83.3 rps18_p2 rps18 2 TTCTGCCCCCATTGTTTCAA CGTTTGTGGATCACTCGGAT 20 20 45.0 50.0 57.9 58.0 0.0 0.0 0.104245 133514 133987 1306-1315 1.000000 83.3 rps18_p3 rps18 3 TCTGCCCCCATTGTTTCAAA CTCGAGGGGCTTATTCAACC 20 20 45.0 55.0 57.9 58.0 0.0 3.9 0.139173 133514 133987 1409-1418 1.000000 82.8 rps18_p4 rps18 4 TCTGCCCCCATTGTTTCAAA GAGGATGCACTCAATCAGGG 20 20 45.0 55.0 57.9 58.0 0.0 0.0 0.129548 133514 133987 952-952 0.500000 59.0 rps18_p5 rps18 5 TTCTGCCCCCATTGTTTCAA GAGGATGCACTCAATCAGGG 20 20 45.0 55.0 57.9 58.0 0.0 0.0 0.129548 133514 133987 953-953 0.500000 59.0 rps12-trnI_p1 rps12-trnI 1 TGGCATTTCTGATTGGCTGT GTTGGGCGCTTTAACCATTC 20 20 45.0 50.0 58.1 58.0 13.3 7.6 0.081713 135627 137159 1598-1620 1.000000 86.9 rps12-trnI_p2 rps12-trnI 2 GTGGCATTTCTGATTGGCTG GTTGGGCGCTTTAACCATTC 20 20 50.0 50.0 57.7 58.0 13.3 7.6 0.298162 135627 137159 1599-1621 1.000000 85.2 rps12-trnI_p3 rps12-trnI 3 TGGCATTTCTGATTGGCTGT AGTTGGGCGCTTTAACCATT 20 20 45.0 45.0 58.1 58.4 13.3 7.6 0.444649 135627 137159 1599-1621 1.000000 84.0 rps12-trnI_p4 rps12-trnI 4 TGGCATTTCTGATTGGCTGT TTGGGCGCTTTAACCATTCA 20 20 45.0 45.0 58.1 58.4 13.3 7.6 0.450520 135627 137159 1597-1619 1.000000 84.0 rps12-trnI_p5 rps12-trnI 5 TGGCATTTCTGATTGGCTGT TGAACCTACGAATTCGCCAA 20 20 45.0 45.0 58.1 57.5 13.3 27.0 0.531573 135627 137159 1624-1646 1.000000 83.2 rps11_p1 rps11 1 TTATTGTTGGGAAGGCCAGG CAGTTTCGTTTTAACATGAGATGGA 20 25 50.0 36.0 57.8 58.1 5.0 0.0 5.332088 141073 142023 1085-1087 1.000000 46.2 rps11_p2 rps11 2 TTATTGTTGGGAAGGCCAGG TCAGTTTCGTTTTAACATGAGATGG 20 25 50.0 36.0 57.8 58.1 5.0 0.0 5.332088 141073 142023 1086-1088 1.000000 46.2 rps11_p3 rps11 3 TTATTGTTGGGAAGGCCAGG AGTTTCGTTTTAACATGAGATGGA 20 24 50.0 33.3 57.8 56.9 5.0 0.0 5.350497 141073 142023 1084-1086 1.000000 46.2 rps11_p4 rps11 4 TTATTGTTGGGAAGGCCAGG CAGTTTCGTTTTAACATGAGATGG 20 24 50.0 37.5 57.8 56.8 5.0 0.0 5.384811 141073 142023 1085-1087 1.000000 46.2 rps11_p5 rps11 5 TTATTGTTGGGAAGGCCAGG GTTTCGTTTTAACATGAGATGGA 20 23 50.0 34.8 57.8 55.5 5.0 0.0 5.704113 141073 142023 1083-1085 1.000000 46.2