Hotspot

rps3

Genome · 93579-94229 · 651 bp

Back to Gephyrocapsa

Pi 0.00205

MarkerSeek score 60.10

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 651
variable_sites: 2
pi: 0.00205
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GCAAGGAAGAGCTTACCGAA	TCCCTTTAATCTTCCCCGGT	50.0	50.0	57.9	58.0	835 bp	1.0	79.2
rps3_p2	GCAAGGAAGAGCTTACCGAA	CCCTTTAATCTTCCCCGGTG	50.0	55.0	57.9	57.9	834 bp	1.0	79.0
rps3_p3	GCAAGGAAGAGCTTACCGAA	AATCTTCCCCGGTGCATTTT	50.0	45.0	57.9	58.1	828 bp	1.0	78.9
rps3_p4	GCAAGGAAGAGCTTACCGAA	CAAATTCGCCAAACACGACA	50.0	45.0	57.9	57.9	875 bp	1.0	78.5
rps3_p5	GCAAGGAAGAGCTTACCGAA	CTTTAATCTTCCCCGGTGCA	50.0	50.0	57.9	57.9	832 bp	1.0	78.5