Hotspot

rbcL

Genome · 11420-12886 · 1467 bp

Back to Gephyrocapsa

Pi 0.000909

MarkerSeek score 58.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: Genome
length_bp: 1467
variable_sites: 2
pi: 0.000909
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	GCATACATCTTAAACCAAGGAGG	AGATATTCCGGGCTAAATGC	43.5	45.0	57.2	55.4	1554 bp	1.0	40.1
rbcL_p2	GCATACATCTTAAACCAAGGAGG	AAGATATTCCGGGCTAAATGC	43.5	42.9	57.2	56.2	1555 bp	1.0	40.1
rbcL_p3	GCATACATCTTAAACCAAGGAGG	GGAAAGAAAAGCATCCTTGAGT	43.5	40.9	57.2	56.9	1612 bp	1.0	40.1
rbcL_p4	GCATACATCTTAAACCAAGGAGG	AGGAAAGAAAAGCATCCTTGAGT	43.5	39.1	57.2	58.3	1613 bp	1.0	40.1
rbcL_p5	GCATACATCTTAAACCAAGGAGG	AGGAAAGAAAAGCATCCTTGAG	43.5	40.9	57.2	56.6	1613 bp	1.0	40.1