Hotspot

psbZ

Genome · 38363-38551 · 189 bp

Back to Gephyrocapsa

Pi 0

MarkerSeek score 20.90

Primer available yes

Label psbZ

Feature Metadata

label_name: psbZ
feature_type: gene
region: Genome
length_bp: 189
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ_p1	CACCACCTGGGAATTCTACG	CACGATACCAAGGTAGTGCC	55.0	55.0	58.0	58.1	1423-1424 bp	1.0	79.7
psbZ_p2	ACGTCAACAATTGGTCGGAA	CACGATACCAAGGTAGTGCC	45.0	55.0	58.0	58.1	1105-1106 bp	1.0	79.6
psbZ_p3	AACAATTGCGCTGTAAACCG	CACGATACCAAGGTAGTGCC	45.0	55.0	58.0	58.1	1452-1453 bp	1.0	79.5
psbZ_p4	TGCTACGCTGATCGACAAAA	CACGATACCAAGGTAGTGCC	45.0	55.0	57.9	58.1	1314-1315 bp	1.0	79.3
psbZ_p5	TTGCTACGCTGATCGACAAA	CACGATACCAAGGTAGTGCC	45.0	55.0	57.9	58.1	1315-1316 bp	1.0	79.3