Hotspot

dfr

Genome · 82199-84037 · 1839 bp

Back to Gephyrocapsa

Pi 0.00254

MarkerSeek score 52.80

Primer available yes

Label dfr

Feature Metadata

label_name: dfr
feature_type: gene
region: Genome
length_bp: 1839
variable_sites: 7
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dfr_p1	AGATTTTCAAAGTCGGCAGT	ACGGGTTGCTAACTCAATGG	40.0	50.0	55.6	58.2	2034 bp	1.0	59.4
dfr_p2	AGATTTTCAAAGTCGGCAGT	CGGGTTGCTAACTCAATGGT	40.0	50.0	55.6	58.2	2033 bp	1.0	59.4
dfr_p3	CCAAAATAAAAGAATTCGCCGC	ACGGGTTGCTAACTCAATGG	40.9	50.0	57.5	58.2	1963 bp	1.0	58.9
dfr_p4	CCAAAATAAAAGAATTCGCCGC	CGGGTTGCTAACTCAATGGT	40.9	50.0	57.5	58.2	1962 bp	1.0	58.9
dfr_p5	AAAGAATTCGCCGCAAAATT	CGGGTTGCTAACTCAATGGT	35.0	50.0	55.4	58.2	1954 bp	1.0	58.3