Hotspot

atpA

Genome · 63337-64836 · 1500 bp

Back to Gephyrocapsa

Pi 0.00178

MarkerSeek score 59.90

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: Genome
length_bp: 1500
variable_sites: 4
pi: 0.00178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TCTTCTTGGAGCAGTTTAAGCT	ACGCCCCATACCCATCATAT	40.9	50.0	57.6	58.3	1721 bp	1.0	58.7
atpA_p2	GCTCATTTCAAGGAGTTAAGGC	ACGCCCCATACCCATCATAT	45.5	50.0	57.6	58.3	1702 bp	1.0	58.6
atpA_p3	AGTACTCTTCTTGGAGCAGT	ACGCCCCATACCCATCATAT	45.0	50.0	55.5	58.3	1726 bp	1.0	57.5
atpA_p4	CTCTTCTTGGAGCAGTTTAAGC	ACGCCCCATACCCATCATAT	45.5	50.0	57.3	58.3	1722 bp	1.0	56.1
atpA_p5	AGTACTCTTCTTGGAGCAGTT	ACGCCCCATACCCATCATAT	42.9	50.0	56.3	58.3	1726 bp	1.0	56.0