Hotspot

psbZ-trnN-GUU

Genome · 38552-38649 · 98 bp

Back to Gephyrocapsa

Pi 0.034

MarkerSeek score 61.70

Primer available yes

Label psbZ-trnN-GUU

Feature Metadata

label_name: psbZ-trnN-GUU
feature_type: igs
region: Genome
length_bp: 98
variable_sites: 5
pi: 0.034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnN-GUU_p1	TATTTTAGCGACGCCTGGTG	AACCGGTTTGTCGTAGGTTC	50.0	50.0	58.4	58.1	250 bp	1.0	77.7
psbZ-trnN-GUU_p2	TATTTTAGCGACGCCTGGTG	CCGGTTTGTCGTAGGTTCAA	50.0	50.0	58.4	58.1	248 bp	1.0	77.7
psbZ-trnN-GUU_p3	TTATTTTAGCGACGCCTGGT	AACCGGTTTGTCGTAGGTTC	45.0	50.0	57.6	58.1	251 bp	1.0	77.4
psbZ-trnN-GUU_p4	TTATTTTAGCGACGCCTGGT	CCGGTTTGTCGTAGGTTCAA	45.0	50.0	57.6	58.1	249 bp	1.0	77.4
psbZ-trnN-GUU_p5	TATTTTAGCGACGCCTGGTG	CCTCAGTAGCTCAGGGGTAG	50.0	60.0	58.4	58.3	284 bp	1.0	76.2