primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	petB-petD	1	ATTACCCGTTGTAGCAGCAG	TTATGGCCCATACCTTTCGC	20	20	50.0	50.0	58.0	58.0	0.0	2.0	0.048516	5584	5665	235-235	1.000000	80.3
petB-petD_p2	petB-petD	2	ATTACCCGTTGTAGCAGCAG	GCACGAAGTTTTGGATCAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.055072	5584	5665	208-208	1.000000	80.3
petB-petD_p3	petB-petD	3	TGACGCGTTTCTATAGTGCC	GCACGAAGTTTTGGATCAGC	20	20	50.0	50.0	58.1	58.0	15.5	0.0	0.113624	5584	5665	239-239	1.000000	79.8
petB-petD_p4	petB-petD	4	TGACGCGTTTCTATAGTGCC	TTATGGCCCATACCTTTCGC	20	20	50.0	50.0	58.1	58.0	15.5	2.0	0.107068	5584	5665	266-266	1.000000	79.7
petB-petD_p5	petB-petD	5	CGCGTTTCTATAGTGCCCAT	TTATGGCCCATACCTTTCGC	20	20	50.0	50.0	58.1	58.0	0.0	2.0	0.158475	5584	5665	263-263	1.000000	79.3
rbcL_p1	rbcL	1	GCATACATCTTAAACCAAGGAGG	AGATATTCCGGGCTAAATGC	23	20	43.5	45.0	57.2	55.4	0.0	0.0	6.405521	11420	12886	1554-1554	1.000000	40.1
rbcL_p2	rbcL	2	GCATACATCTTAAACCAAGGAGG	AAGATATTCCGGGCTAAATGC	23	21	43.5	42.9	57.2	56.2	0.0	0.0	6.605273	11420	12886	1555-1555	1.000000	40.1
rbcL_p3	rbcL	3	GCATACATCTTAAACCAAGGAGG	GGAAAGAAAAGCATCCTTGAGT	23	22	43.5	40.9	57.2	56.9	0.0	0.0	6.884815	11420	12886	1612-1612	1.000000	40.1
rbcL_p4	rbcL	4	GCATACATCTTAAACCAAGGAGG	AGGAAAGAAAAGCATCCTTGAGT	23	23	43.5	39.1	57.2	58.3	0.0	0.0	7.023584	11420	12886	1613-1613	1.000000	40.1
rbcL_p5	rbcL	5	GCATACATCTTAAACCAAGGAGG	AGGAAAGAAAAGCATCCTTGAG	23	22	43.5	40.9	57.2	56.6	0.0	0.0	7.157566	11420	12886	1613-1613	1.000000	40.1
rbcS_p1	rbcS	1	TTCTAGCACGTAACGAAGGC	GAGCTTGGTACTACAGCACC	20	20	50.0	55.0	58.0	58.0	0.0	13.1	0.024842	12973	13392	1192-1192	1.000000	80.3
rbcS_p2	rbcS	2	GTGGTCCTCTTCAAACTGCA	GAGCTTGGTACTACAGCACC	20	20	50.0	55.0	58.0	58.0	0.0	13.1	0.044222	12973	13392	1120-1120	1.000000	80.2
rbcS_p3	rbcS	3	TGGATGCGTATGTCAGGTTG	GAGCTTGGTACTACAGCACC	20	20	50.0	55.0	58.0	58.0	0.0	13.1	0.034305	12973	13392	1526-1526	1.000000	80.1
rbcS_p4	rbcS	4	GGATGCGTATGTCAGGTTGT	GAGCTTGGTACTACAGCACC	20	20	50.0	55.0	58.0	58.0	0.0	13.1	0.035716	12973	13392	1525-1525	1.000000	80.1
rbcS_p5	rbcS	5	GTGGATGCGTATGTCAGGTT	GAGCTTGGTACTACAGCACC	20	20	50.0	55.0	58.0	58.0	0.0	13.1	0.035716	12973	13392	1527-1527	1.000000	80.1
rrnS-psbI_p1	rrnS-psbI	1	GCATGTGTTAAGCATACCGC	CCATCTAGAAACCCGAACCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.169390	20813	21053	335-335	1.000000	79.4
rrnS-psbI_p2	rrnS-psbI	2	AAACTCTCGTTCGACTTGCA	CCATCTAGAAACCCGAACCG	20	20	45.0	55.0	57.8	58.1	0.0	0.0	0.283020	20813	21053	352-352	1.000000	78.4
rrnS-psbI_p3	rrnS-psbI	3	TGTGTTAAGCATACCGCCAG	CCATCTAGAAACCCGAACCG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.331127	20813	21053	332-332	1.000000	78.1
rrnS-psbI_p4	rrnS-psbI	4	ACTCTCGTTCGACTTGCATG	CCATCTAGAAACCCGAACCG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.361181	20813	21053	350-350	1.000000	77.8
rrnS-psbI_p5	rrnS-psbI	5	CTCTCGTTCGACTTGCATGT	CCATCTAGAAACCCGAACCG	20	20	50.0	55.0	58.3	58.1	4.7	0.0	0.361181	20813	21053	349-349	1.000000	77.8
psbZ_p1	psbZ	1	CACCACCTGGGAATTCTACG	CACGATACCAAGGTAGTGCC	20	20	55.0	55.0	58.0	58.1	5.2	5.1	0.088243	38363	38551	1423-1424	1.000000	79.7
psbZ_p2	psbZ	2	ACGTCAACAATTGGTCGGAA	CACGATACCAAGGTAGTGCC	20	20	45.0	55.0	58.0	58.1	10.8	5.1	0.107771	38363	38551	1105-1106	1.000000	79.6
psbZ_p3	psbZ	3	AACAATTGCGCTGTAAACCG	CACGATACCAAGGTAGTGCC	20	20	45.0	55.0	58.0	58.1	9.7	5.1	0.108955	38363	38551	1452-1453	1.000000	79.5
psbZ_p4	psbZ	4	TGCTACGCTGATCGACAAAA	CACGATACCAAGGTAGTGCC	20	20	45.0	55.0	57.9	58.1	0.0	5.1	0.137184	38363	38551	1314-1315	1.000000	79.3
psbZ_p5	psbZ	5	TTGCTACGCTGATCGACAAA	CACGATACCAAGGTAGTGCC	20	20	45.0	55.0	57.9	58.1	0.0	5.1	0.137184	38363	38551	1315-1316	1.000000	79.3
psbZ-trnN-GUU_p1	psbZ-trnN-GUU	1	TATTTTAGCGACGCCTGGTG	AACCGGTTTGTCGTAGGTTC	20	20	50.0	50.0	58.4	58.1	17.8	15.9	0.482880	38552	38649	250-250	1.000000	77.7
psbZ-trnN-GUU_p2	psbZ-trnN-GUU	2	TATTTTAGCGACGCCTGGTG	CCGGTTTGTCGTAGGTTCAA	20	20	50.0	50.0	58.4	58.1	17.8	0.0	0.484185	38552	38649	248-248	1.000000	77.7
psbZ-trnN-GUU_p3	psbZ-trnN-GUU	3	TTATTTTAGCGACGCCTGGT	AACCGGTTTGTCGTAGGTTC	20	20	45.0	50.0	57.6	58.1	17.8	15.9	0.520188	38552	38649	251-251	1.000000	77.4
psbZ-trnN-GUU_p4	psbZ-trnN-GUU	4	TTATTTTAGCGACGCCTGGT	CCGGTTTGTCGTAGGTTCAA	20	20	45.0	50.0	57.6	58.1	17.8	0.0	0.521493	38552	38649	249-249	1.000000	77.4
psbZ-trnN-GUU_p5	psbZ-trnN-GUU	5	TATTTTAGCGACGCCTGGTG	CCTCAGTAGCTCAGGGGTAG	20	20	50.0	60.0	58.4	58.3	17.8	0.0	0.654148	38552	38649	284-284	1.000000	76.2
atpA_p1	atpA	1	TCTTCTTGGAGCAGTTTAAGCT	ACGCCCCATACCCATCATAT	22	20	40.9	50.0	57.6	58.3	0.0	0.0	2.689092	63337	64836	1721-1721	1.000000	58.7
atpA_p2	atpA	2	GCTCATTTCAAGGAGTTAAGGC	ACGCCCCATACCCATCATAT	22	20	45.5	50.0	57.6	58.3	3.4	0.0	2.694106	63337	64836	1702-1702	1.000000	58.6
atpA_p3	atpA	3	AGTACTCTTCTTGGAGCAGT	ACGCCCCATACCCATCATAT	20	20	45.0	50.0	55.5	58.3	0.0	0.0	2.832586	63337	64836	1726-1726	1.000000	57.5
atpA_p4	atpA	4	CTCTTCTTGGAGCAGTTTAAGC	ACGCCCCATACCCATCATAT	22	20	45.5	50.0	57.3	58.3	0.0	0.0	3.008023	63337	64836	1722-1722	1.000000	56.1
atpA_p5	atpA	5	AGTACTCTTCTTGGAGCAGTT	ACGCCCCATACCCATCATAT	21	20	42.9	50.0	56.3	58.3	0.0	0.0	3.027788	63337	64836	1726-1726	1.000000	56.0
trnR-ACG-trnS-UGA_p1	trnR-ACG-trnS-UGA	1	AAAGGGCTTGTAGCTCAGTG	GGTTCGAATCCCACCTTCTC	20	20	50.0	55.0	57.8	58.0	0.9	0.0	0.216716	65153	65478	425-425	1.000000	78.6
trnR-ACG-trnS-UGA_p2	trnR-ACG-trnS-UGA	2	AAAGGGCTTGTAGCTCAGTG	GTTCGAATCCCACCTTCTCC	20	20	50.0	55.0	57.8	58.0	0.9	0.0	0.216716	65153	65478	424-424	1.000000	78.6
trnR-ACG-trnS-UGA_p3	trnR-ACG-trnS-UGA	3	ACAAAGGGCTTGTAGCTCAG	GTTCGAATCCCACCTTCTCC	20	20	50.0	55.0	57.8	58.0	0.9	0.0	0.216716	65153	65478	426-426	1.000000	78.6
trnR-ACG-trnS-UGA_p4	trnR-ACG-trnS-UGA	4	CAAAGGGCTTGTAGCTCAGT	GGTTCGAATCCCACCTTCTC	20	20	50.0	55.0	57.8	58.0	0.9	0.0	0.216716	65153	65478	426-426	1.000000	78.6
trnR-ACG-trnS-UGA_p5	trnR-ACG-trnS-UGA	5	CAAAGGGCTTGTAGCTCAGT	GTTCGAATCCCACCTTCTCC	20	20	50.0	55.0	57.8	58.0	0.9	0.0	0.216716	65153	65478	425-425	1.000000	78.6
ycf80_p1	ycf80	1	ATTCCAAAACAGCCCCTTGG	CCCCTAACCGCGGATTTATC	20	20	50.0	55.0	58.6	58.2	0.0	35.9	0.844323	70713	72173	1652-1652	1.000000	73.3
ycf80_p2	ycf80	2	CGATTCCAAAACAGCCCCTT	CCCCTAACCGCGGATTTATC	20	20	50.0	55.0	58.7	58.2	0.0	35.9	0.938587	70713	72173	1654-1654	1.000000	72.6
ycf80_p3	ycf80	3	GATTCCAAAACAGCCCCTTG	CCCCTAACCGCGGATTTATC	20	20	50.0	55.0	57.3	58.2	0.0	35.9	0.944679	70713	72173	1653-1653	1.000000	72.5
ycf80_p4	ycf80	4	TGTCGATTCCAAAACAGCCC	CCCCTAACCGCGGATTTATC	20	20	50.0	55.0	58.8	58.2	0.0	35.9	0.951497	70713	72173	1657-1657	1.000000	72.5
ycf80_p5	ycf80	5	ATTCCAAAACAGCCCCTTGG	CCCTAACCGCGGATTTATCA	20	20	50.0	50.0	58.6	57.5	0.0	35.9	1.185685	70713	72173	1651-1651	1.000000	70.6
rbcR-trnE-UUC_p1	rbcR-trnE-UUC	1	ACTTCAGCAGCCTCAACAAA	ATGTCCTAGGCCTCTAGACG	20	20	45.0	55.0	57.9	57.7	0.0	9.7	0.337072	76671	76852	240-240	1.000000	78.0
rbcR-trnE-UUC_p2	rbcR-trnE-UUC	2	ACTTCAGCAGCCTCAACAAA	CCTAGGCCTCTAGACGATGG	20	20	45.0	60.0	57.9	58.5	0.0	9.7	0.516870	76671	76852	236-236	1.000000	76.5
rbcR-trnE-UUC_p3	rbcR-trnE-UUC	3	ACTTCAGCAGCCTCAACAAA	CTAGGCCTCTAGACGATGGG	20	20	45.0	60.0	57.9	58.5	0.0	9.7	0.516870	76671	76852	235-235	1.000000	76.5
rbcR-trnE-UUC_p4	rbcR-trnE-UUC	4	ACTTCAGCAGCCTCAACAAA	AAAGGGAGATGTCCTAGGCC	20	20	45.0	55.0	57.9	58.5	0.0	9.1	0.544402	76671	76852	248-248	1.000000	76.3
rbcR-trnE-UUC_p5	rbcR-trnE-UUC	5	ACTTCAGCAGCCTCAACAAA	TCCTAGGCCTCTAGACGATG	20	20	45.0	55.0	57.9	57.4	0.0	9.7	0.627552	76671	76852	237-237	1.000000	75.7
petG-secA_p1	petG-secA	1	AGAGGAAACCAATTTGGGCT	AATCGTATCCGTCATCAGGT	20	20	45.0	45.0	57.3	56.1	19.4	0.0	2.564496	79175	79225	141-141	1.000000	62.3
petG-secA_p2	petG-secA	2	AGAGGAAACCAATTTGGGCT	AAATCGTATCCGTCATCAGGT	20	21	45.0	42.9	57.3	56.9	19.4	0.0	2.799975	79175	79225	142-142	1.000000	60.4
petG-secA_p3	petG-secA	3	AAGAGGAAACCAATTTGGGCT	AATCGTATCCGTCATCAGGT	21	20	42.9	45.0	58.0	56.1	19.4	0.0	2.885786	79175	79225	142-142	1.000000	59.7
petG-secA_p4	petG-secA	4	AAGAGGAAACCAATTTGGGCT	AAATCGTATCCGTCATCAGGT	21	21	42.9	42.9	58.0	56.9	19.4	0.0	3.121264	79175	79225	143-143	1.000000	57.8
petG-secA_p5	petG-secA	5	GAAGAGGAAACCAATTTGGGC	AATCGTATCCGTCATCAGGT	21	20	47.6	45.0	57.7	56.1	19.4	0.0	3.181364	79175	79225	143-143	1.000000	57.3
secA_p1	secA	1	AGAGGAAACCAATTTGGGCT	CTGCTCCGTCTCCCTATAGA	20	20	45.0	55.0	57.3	57.4	19.4	0.0	1.272685	79226	81871	2825-2825	1.000000	70.0
secA_p2	secA	2	AAGAGGAAACCAATTTGGGCT	CTGCTCCGTCTCCCTATAGA	21	20	42.9	55.0	58.0	57.4	19.4	0.0	1.593974	79226	81871	2826-2826	1.000000	67.5
secA_p3	secA	3	AGAGGAAACCAATTTGGGCT	GCTGCTCCGTCTCCCTATA	20	19	45.0	57.9	57.3	58.0	19.4	0.0	1.740211	79226	81871	2826-2826	1.000000	66.3
secA_p4	secA	4	AGAGGAAACCAATTTGGGCT	TGCTCCGTCTCCCTATAGAA	20	20	45.0	50.0	57.3	57.0	19.4	0.0	1.746737	79226	81871	2824-2824	1.000000	66.3
secA_p5	secA	5	AGAGGAAACCAATTTGGGCT	GCTGCTCCGTCTCCCTATAG	20	20	45.0	60.0	57.3	59.1	19.4	0.0	1.817074	79226	81871	2826-2826	1.000000	65.7
dfr_p1	dfr	1	AGATTTTCAAAGTCGGCAGT	ACGGGTTGCTAACTCAATGG	20	20	40.0	50.0	55.6	58.2	0.0	0.0	2.604359	82199	84037	2034-2034	1.000000	59.4
dfr_p2	dfr	2	AGATTTTCAAAGTCGGCAGT	CGGGTTGCTAACTCAATGGT	20	20	40.0	50.0	55.6	58.2	0.0	0.0	2.604359	82199	84037	2033-2033	1.000000	59.4
dfr_p3	dfr	3	CCAAAATAAAAGAATTCGCCGC	ACGGGTTGCTAACTCAATGG	22	20	40.9	50.0	57.5	58.2	0.0	0.0	2.677957	82199	84037	1963-1963	1.000000	58.9
dfr_p4	dfr	4	CCAAAATAAAAGAATTCGCCGC	CGGGTTGCTAACTCAATGGT	22	20	40.9	50.0	57.5	58.2	0.0	0.0	2.677957	82199	84037	1962-1962	1.000000	58.9
dfr_p5	dfr	5	AAAGAATTCGCCGCAAAATT	CGGGTTGCTAACTCAATGGT	20	20	35.0	50.0	55.4	58.2	0.0	0.0	2.749398	82199	84037	1954-1954	1.000000	58.3
rps3_p1	rps3	1	GCAAGGAAGAGCTTACCGAA	TCCCTTTAATCTTCCCCGGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.119292	93579	94229	835-835	1.000000	79.2
rps3_p2	rps3	2	GCAAGGAAGAGCTTACCGAA	CCCTTTAATCTTCCCCGGTG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.147544	93579	94229	834-834	1.000000	79.0
rps3_p3	rps3	3	GCAAGGAAGAGCTTACCGAA	AATCTTCCCCGGTGCATTTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.157571	93579	94229	828-828	1.000000	78.9
rps3_p4	rps3	4	GCAAGGAAGAGCTTACCGAA	CAAATTCGCCAAACACGACA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.207237	93579	94229	875-875	1.000000	78.5
rps3_p5	rps3	5	GCAAGGAAGAGCTTACCGAA	CTTTAATCTTCCCCGGTGCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.217545	93579	94229	832-832	1.000000	78.5
secY_p1	secY	1	TTTTAAATAATGCGCGGGCG	AGTCGCGTATTAAACCATTAACT	20	23	45.0	34.8	58.2	56.4	20.2	5.8	4.800984	97417	98685	1470-1470	1.000000	41.8
secY_p2	secY	2	GGCGACAATCGATGGTCTTA	AGTCGCGTATTAAACCATTAACT	20	23	50.0	34.8	57.8	56.4	26.5	5.8	4.848768	97417	98685	1454-1454	1.000000	41.4
secY_p3	secY	3	TTTTAAATAATGCGCGGGCG	AGTCGCGTATTAAACCATTAAC	20	22	45.0	36.4	58.2	55.0	20.2	5.8	5.216763	97417	98685	1470-1470	1.000000	40.2
secY_p4	secY	4	TTTTAAATAATGCGCGGGCG	GTCGCGTATTAAACCATTAACT	20	22	45.0	36.4	58.2	55.0	20.2	5.8	5.216763	97417	98685	1469-1469	1.000000	40.2
secY_p5	secY	5	GGCGACAATCGATGGTCTTA	GTCGCGTATTAAACCATTAACT	20	22	50.0	36.4	57.8	55.0	26.5	5.8	5.264548	97417	98685	1453-1453	1.000000	40.2
rpoA_p1	rpoA	1	CTGTCCCTCACAATGGCTG	TGATACCAGTCCCAGGTTGT	19	20	57.9	50.0	58.1	58.0	0.0	0.0	1.174749	99848	100777	1135-1135	1.000000	71.0
rpoA_p2	rpoA	2	CTGTCCCTCACAATGGCTG	GCTTCTTTTCGCCTTCCAAC	19	20	57.9	50.0	58.1	57.9	0.0	0.0	1.187764	99848	100777	1097-1097	1.000000	70.9
rpoA_p3	rpoA	3	TACACCTGTCCCTCACAATG	TGATACCAGTCCCAGGTTGT	20	20	50.0	50.0	56.9	58.0	0.0	0.0	1.188823	99848	100777	1140-1140	1.000000	70.8
rpoA_p4	rpoA	4	TACACCTGTCCCTCACAATG	GCTTCTTTTCGCCTTCCAAC	20	20	50.0	50.0	56.9	57.9	0.0	0.0	1.201839	99848	100777	1102-1102	1.000000	70.7
rpoA_p5	rpoA	5	CACCTGTCCCTCACAATGG	TGATACCAGTCCCAGGTTGT	19	20	57.9	50.0	57.7	58.0	0.0	0.0	1.293492	99848	100777	1138-1138	1.000000	70.0
ycf39_p1	ycf39	1	GCGGTGCTCTAACCAATTGA	TGATTTCTGGGGCTGTTTTT	20	20	50.0	40.0	58.6	55.7	7.1	0.0	2.838921	104210	105130	1156-1156	1.000000	57.5
ycf39_p2	ycf39	2	GCGGTGCTCTAACCAATTGA	AATGATTTCTGGGGCTGTTT	20	20	50.0	40.0	58.6	55.5	7.1	0.0	3.077225	104210	105130	1158-1158	1.000000	55.6
ycf39_p3	ycf39	3	GCGGTGCTCTAACCAATTGA	ATGATTTCTGGGGCTGTTTT	20	20	50.0	40.0	58.6	55.5	7.1	0.0	3.077225	104210	105130	1157-1157	1.000000	55.6
ycf39_p4	ycf39	4	GCGGTGCTCTAACCAATTGA	TTCTGGGGCTGTTTTTACAA	20	20	50.0	40.0	58.6	55.4	7.1	0.0	3.193395	104210	105130	1152-1152	1.000000	54.7
ycf39_p5	ycf39	5	GCGGTGCTCTAACCAATTGA	TTTCTGGGGCTGTTTTTACA	20	20	50.0	40.0	58.6	55.4	7.1	0.0	3.193395	104210	105130	1153-1153	1.000000	54.7