Hotspot

ycf1

SSC · 118539-123011 · 4473 bp

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Pi 0.00328

MarkerSeek score 55.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4473
variable_sites: 22
pi: 0.00328
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGACGATTCACGAACAAT	AGGTGAAAAAGAGATTTTTACAAGT	45.0	28.0	57.9	55.6	226 bp	1.0	41.4
ycf1_p2	AACGGACGATTCACGAACAA	AGGTGAAAAAGAGATTTTTACAAGT	45.0	28.0	58.1	55.6	227 bp	1.0	41.4
ycf1_p3	AGAACGGACGATTCACGAAC	AGGTGAAAAAGAGATTTTTACAAGT	50.0	28.0	58.3	55.6	229 bp	1.0	41.4
ycf1_p4	ACGGACGATTCACGAACAAT	GGTGAAAAAGAGATTTTTACAAGT	45.0	29.2	57.9	54.2	225 bp	1.0	41.4
ycf1_p5	ACGGACGATTCACGAACAAT	AGGTGAAAAAGAGATTTTTACAAGTT	45.0	26.9	57.9	56.2	226 bp	1.0	41.4