Hotspot

rpoA

LSC · 78877-79896 · 1020 bp

Back to Geodorum

Pi 0.00261

MarkerSeek score 41.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 4
pi: 0.00261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGGACCGTGTCGAAGATTAA	TGATTCAATGATCTGATCCAA	45.0	33.3	56.6	52.6	1154 bp	1.0	40.3
rpoA_p2	ATGGACCGTGTCGAAGATTA	TGATTCAATGATCTGATCCAA	45.0	33.3	56.4	52.6	1155 bp	1.0	40.3
rpoA_p3	TATGGACCGTGTCGAAGATT	TGATTCAATGATCTGATCCAA	45.0	33.3	56.4	52.6	1156 bp	1.0	40.3
rpoA_p4	TGGACCGTGTCGAAGATTAA	ATGATTCAATGATCTGATCCA	45.0	33.3	56.6	52.3	1155 bp	1.0	40.3
rpoA_p5	TGGACCGTGTCGAAGATTAAA	TGATTCAATGATCTGATCCAA	42.9	33.3	57.3	52.6	1154 bp	1.0	40.3