Hotspot

accD

LSC · 57783-59231 · 1449 bp

Back to Geodorum

Pi 0.00184

MarkerSeek score 60.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1449
variable_sites: 4
pi: 0.00184
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAGAAAGAATCT	AGTTCTCGCTAAATAGAAAGAACT	45.0	33.3	55.6	55.9	1588-1595 bp	1.0	40.4
accD_p2	CCTAAAATAGGGGGCAGAAAGA	AGTTCTCGCTAAATAGAAAGAACT	45.5	33.3	57.5	55.9	1594-1601 bp	1.0	40.4
accD_p3	TCCTAAAATAGGGGGCAGAAAG	AGTTCTCGCTAAATAGAAAGAACT	45.5	33.3	57.5	55.9	1595-1602 bp	1.0	40.4
accD_p4	GGTTTTCCTAAAATAGGGGGCA	AGTTCTCGCTAAATAGAAAGAACT	45.5	33.3	58.3	55.9	1600-1607 bp	1.0	40.3
accD_p5	GGTTTTCCTAAAATAGGGGGC	AGTTCTCGCTAAATAGAAAGAACT	47.6	33.3	56.5	55.9	1600-1607 bp	1.0	40.3