Hotspot

psaC-rps15

SSC · 114823-117904 · 3082 bp

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Pi 0.00346

MarkerSeek score 58.90

Primer available yes

Label psaC-rps15

Feature Metadata

label_name: psaC-rps15
feature_type: igs
region: SSC
length_bp: 3082
variable_sites: 16
pi: 0.00346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-rps15_p1	TCCAAAAAGTTCAGTAAGAGA	TTGGGAATGGGATCTTTGGC	33.3	50.0	52.3	58.1	266 bp	1.0	40.9
psaC-rps15_p2	TTCCAAAAAGTTCAGTAAGAGA	TTGGGAATGGGATCTTTGGC	31.8	50.0	53.2	58.1	267 bp	1.0	40.9
psaC-rps15_p3	TCCAAAAAGTTCAGTAAGAGA	AATGGGATCTTTGGCGTCAG	33.3	50.0	52.3	58.2	261 bp	1.0	40.9
psaC-rps15_p4	TCCAAAAAGTTCAGTAAGAGA	GAATGGGATCTTTGGCGTCA	33.3	50.0	52.3	58.2	262 bp	1.0	40.9
psaC-rps15_p5	TTCCAAAAAGTTCAGTAAGAGA	AATGGGATCTTTGGCGTCAG	31.8	50.0	53.2	58.2	262 bp	1.0	40.9