Hotspot

ccsA-psaC

SSC · 112680-114576 · 1897 bp

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Pi 0.00668

MarkerSeek score 49.10

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1897
variable_sites: 19
pi: 0.00668
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TTTTACCCAAGGTTTCGCCA	AGATGTGAATCCGCTTGTCC	45.0	50.0	57.9	58.0	2639-2645 bp	1.0	80.1
ccsA-psaC_p2	TTTTACCCAAGGTTTCGCCA	AGGATGTACCCAATGCGTTC	45.0	50.0	57.9	58.0	2760-2766 bp	1.0	80.0
ccsA-psaC_p3	TTTTACCCAAGGTTTCGCCA	GCTTGTCCCACGGATGTATT	45.0	50.0	57.9	58.0	2738-2744 bp	1.0	80.0
ccsA-psaC_p4	TTTTACCCAAGGTTTCGCCA	GATTCGTCTGGTGTCGACAA	45.0	50.0	57.9	57.9	2914-2920 bp	1.0	79.8
ccsA-psaC_p5	TTTTACCCAAGGTTTCGCCA	TTGATTCGTCTGGTGTCGAC	45.0	50.0	57.9	57.9	2916-2922 bp	1.0	79.8