Hotspot

trnL-UAG-ccsA

SSC · 111562-111701 · 140 bp

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Pi 0.0238
MarkerSeek score 67.70
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
140
variable_sites
5
pi
0.0238
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA TCACAATTGAGATGACCGAA 50.0 40.0 57.9 54.7 264 bp 1.0 54.2
trnL-UAG-ccsA_p2 AAATTGGTAGACACGCTGCT TCACAATTGAGATGACCGAA 45.0 40.0 57.8 54.7 271 bp 1.0 53.6
trnL-UAG-ccsA_p3 GGTAGACACGCTGCTCTTAG TCACAATTGAGATGACCGAA 55.0 40.0 57.8 54.7 266 bp 1.0 53.6
trnL-UAG-ccsA_p4 GTAGACACGCTGCTCTTAGG TCACAATTGAGATGACCGAA 55.0 40.0 57.8 54.7 265 bp 1.0 53.6
trnL-UAG-ccsA_p5 ATTTAATGCAAGCCGCCATG TCACAATTGAGATGACCGAA 45.0 40.0 57.8 54.7 294 bp 0.666667 38.7