Hotspot

trnN-GUU-rpl32

IRb · 110142-110620 · 479 bp

Back to Geodorum

Pi 0.00974

MarkerSeek score 57.80

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 479
variable_sites: 6
pi: 0.00974
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.1	57.3	599-622 bp	1.0	52.0
trnN-GUU-rpl32_p2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.8	57.3	605-628 bp	1.0	51.7
trnN-GUU-rpl32_p3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.2	57.3	618-641 bp	1.0	51.6
trnN-GUU-rpl32_p4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	58.1	56.2	606-629 bp	1.0	51.3
trnN-GUU-rpl32_p5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	55.0	43.5	57.8	57.3	617-640 bp	1.0	51.2