Hotspot

clpP-psbB

LSC · 72246-72930 · 685 bp

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Pi 0.00876

MarkerSeek score 57.80

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 685
variable_sites: 9
pi: 0.00876
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	858-881 bp	1.0	80.7
clpP-psbB_p2	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	809-832 bp	1.0	79.6
clpP-psbB_p3	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	45.0	55.0	58.3	57.9	807-830 bp	1.0	78.7
clpP-psbB_p4	TCCAGGACTCCGAAAAGGTA	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.7	57.9	829-852 bp	1.0	78.4
clpP-psbB_p5	CAAGCTGCATCTTCCTCTCC	GGCCCAACCAGAAACTAGAG	55.0	55.0	58.3	57.9	846-869 bp	1.0	78.2