Hotspot

atpB-rbcL

LSC · 54517-55501 · 985 bp

Back to Geodorum

Pi 0.00203

MarkerSeek score 62.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 3
pi: 0.00203
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	ACCAGCTTTAAATCCAACGC	45.0	45.0	57.9	57.0	1097-1138 bp	1.0	72.8
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	AGCTTTAAATCCAACGCTTGC	45.0	42.9	57.9	58.1	1094-1135 bp	1.0	72.4
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	GCTTTAAATCCAACGCTTGCT	45.0	42.9	57.9	58.1	1093-1134 bp	1.0	72.4
atpB-rbcL_p4	CAGGATCGGAAGTAGTGGGA	ACCAGCTTTAAATCCAACGC	55.0	45.0	58.2	57.0	1041-1082 bp	1.0	72.2
atpB-rbcL_p5	ACCACAGGATCGGAAGTAGT	ACCAGCTTTAAATCCAACGC	50.0	45.0	57.8	57.0	1045-1086 bp	1.0	72.1