Hotspot

trnF-GAA-trnV-UAC

LSC · 48661-51542 · 2882 bp

Back to Geodorum

Pi 0.00324

MarkerSeek score 34.40

Primer available yes

Label trnF-GAA-trnV-UAC

Feature Metadata

label_name: trnF-GAA-trnV-UAC
feature_type: igs
region: LSC
length_bp: 2882
variable_sites: 14
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnV-UAC_p1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	2949-2961 bp	1.0	78.4
trnF-GAA-trnV-UAC_p2	CCTCGTGTCACCAGTTCAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	2948-2960 bp	1.0	75.6
trnF-GAA-trnV-UAC_p3	AATCCTCGTGTCACCAGTTC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	2952-2964 bp	1.0	75.1
trnF-GAA-trnV-UAC_p4	CCTCGTGTCACCAGTTCAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.1	58.7	2937-2949 bp	1.0	74.5
trnF-GAA-trnV-UAC_p5	ATCCTCGTGTCACCAGTTCA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.7	57.8	2951-2963 bp	1.0	73.5