Hotspot

psbM-trnD-GUC

LSC · 29491-30602 · 1112 bp

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Pi 0.0054

MarkerSeek score 62.20

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1112
variable_sites: 9
pi: 0.0054
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TTGGCTGACTGTTTTGACGT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1296 bp	1.0	78.0
psbM-trnD-GUC_p2	ATGAACAGCGCAGTAGCAAT	CCATGCATATGGCTGGGATT	45.0	50.0	58.3	58.1	1244 bp	1.0	78.0
psbM-trnD-GUC_p3	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1274 bp	1.0	77.8
psbM-trnD-GUC_p4	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1222 bp	1.0	77.8
psbM-trnD-GUC_p5	TGAACAGCGCAGTAGCAATA	CCATGCATATGGCTGGGATT	45.0	50.0	57.6	58.1	1243 bp	1.0	77.0