Hotspot

rps16-trnQ-UUG

LSC · 5759-6753 · 995 bp

Back to Geodorum

Pi 0.00804
MarkerSeek score 58.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
995
variable_sites
12
pi
0.00804
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GACCAAATCAAAATGGGGCG 45.0 50.0 58.1 58.0 1130-1143 bp 1.0 80.0
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GACCAAATCAAAATGGGGCG 50.0 50.0 58.2 58.0 1141-1154 bp 1.0 79.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA CAAATCAAAATGGGGCGTGG 45.0 50.0 58.1 58.3 1127-1140 bp 1.0 77.9
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CCAAATCAAAATGGGGCGTG 45.0 50.0 58.1 58.3 1128-1141 bp 1.0 77.9
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC CAAATCAAAATGGGGCGTGG 50.0 50.0 58.2 58.3 1138-1151 bp 1.0 77.4