Hotspot

ycf4

LSC · 58922-59476 · 555 bp

Back to Gentianopsis

Pi 0.0024

MarkerSeek score 58.90

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 2
pi: 0.0024
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	AGGTTCACATCAAACTAGTGCT	GGGCGATTCTTGCGTTTTTA	40.9	45.0	57.6	57.4	719-722 bp	1.0	56.7
ycf4_p2	AGGTTCACATCAAACTAGTGCT	AAGCGGGAATCTACCAGTTC	40.9	50.0	57.6	57.3	689-692 bp	1.0	56.2
ycf4_p3	AGGTTCACATCAAACTAGTGCT	AGAAAAGGGGCGATTCTTGC	40.9	50.0	57.6	58.8	726-729 bp	1.0	55.1
ycf4_p4	AGGTTCACATCAAACTAGTGCT	AAAGCGGGAATCTACCAGTTC	40.9	47.6	57.6	58.0	690-693 bp	1.0	53.6
ycf4_p5	GGTTCACATCAAACTAGTGCT	GGGCGATTCTTGCGTTTTTA	42.9	45.0	56.2	57.4	718-721 bp	1.0	53.0