Hotspot

rpoC1

LSC · 19022-21810 · 2789 bp

Back to Gentianopsis

Pi 0.000717

MarkerSeek score 56.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2789
variable_sites: 3
pi: 0.000717
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGGCCTGAATTTGCACT	AGGAAGGAAGCTTAATCGCA	50.0	45.0	58.2	56.9	2875-2879 bp	1.0	70.1
rpoC1_p2	GAGAGGCCTGAATTTGCACT	GAGGAAGGAAGCTTAATCGCA	50.0	47.6	58.2	58.1	2876-2880 bp	1.0	70.0
rpoC1_p3	TTTGCGGAAAACCTCCCTTT	AGGAAGGAAGCTTAATCGCA	45.0	45.0	58.2	56.9	2946-2950 bp	1.0	69.7
rpoC1_p4	TTTGCGGAAAACCTCCCTTT	GAGGAAGGAAGCTTAATCGCA	45.0	47.6	58.2	58.1	2947-2951 bp	1.0	69.6
rpoC1_p5	GAGAGGCCTGAATTTGCACT	AGAGGAAGGAAGCTTAATCGC	50.0	47.6	58.2	57.8	2877-2881 bp	1.0	69.3