Hotspot

rpl16

LSC · 80168-81352 · 1185 bp

Back to Gentianopsis

Pi 0.00584

MarkerSeek score 52.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1185
variable_sites: 10
pi: 0.00584
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTTTAAATGCAAAGGACAAAGA	ACTGACCCTTTGATAAAATTGCT	30.4	34.8	55.0	56.7	1277-1320 bp	1.0	43.3
rpl16_p2	AAATGCAAAGGACAAAGAAAA	ACTGACCCTTTGATAAAATTGCT	28.6	34.8	53.0	56.7	1272-1315 bp	1.0	43.3
rpl16_p3	AATGCAAAGGACAAAGAAAAA	ACTGACCCTTTGATAAAATTGCT	28.6	34.8	53.0	56.7	1271-1314 bp	1.0	43.3
rpl16_p4	AAATGCAAAGGACAAAGAAAAA	ACTGACCCTTTGATAAAATTGCT	27.3	34.8	53.8	56.7	1272-1315 bp	1.0	43.3
rpl16_p5	TCTTTAAATGCAAAGGACAAAGAA	ACTGACCCTTTGATAAAATTGCT	29.2	34.8	55.7	56.7	1277-1320 bp	1.0	43.3