Hotspot

ccsA

SSC · 112038-113012 · 975 bp

Back to Gentianopsis

Pi 0.00342

MarkerSeek score 58.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 5
pi: 0.00342
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTAGCGGCATGTCATCTT	GCACATACTCTGTTGTAGTCGT	50.0	45.5	57.8	58.2	1131 bp	1.0	61.2
ccsA_p2	GAGTAGCGGCATGTCATCTT	TGCACATACTCTGTTGTAGTCG	50.0	45.5	57.8	58.2	1132 bp	1.0	61.2
ccsA_p3	GAGTAGCGGCATGTCATCTT	ACGATTCATGTCAGTTCACACA	50.0	40.9	57.8	58.3	1165 bp	1.0	60.3
ccsA_p4	GAGTAGCGGCATGTCATCTT	CGATTCATGTCAGTTCACACA	50.0	42.9	57.8	56.6	1164 bp	1.0	59.1
ccsA_p5	GAGTAGCGGCATGTCATCTT	ACGATTCATGTCAGTTCACAC	50.0	42.9	57.8	56.6	1165 bp	1.0	59.1