Hotspot

ndhF-rpl32

SSC · 110184-110947 · 764 bp

Back to Gentianopsis

Pi 0.00524

MarkerSeek score 59.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 764
variable_sites: 6
pi: 0.00524
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACGGGAAGTGGAATGAA	AAGCGGTCCAATATCCCTTC	50.0	50.0	57.8	57.4	904-927 bp	1.0	73.9
ndhF-rpl32_p2	ACGGGAAGTGGAATGAAAGG	AAGCGGTCCAATATCCCTTC	50.0	50.0	57.8	57.4	901-924 bp	1.0	73.9
ndhF-rpl32_p3	CGGGAAGTGGAATGAAAGGT	AAGCGGTCCAATATCCCTTC	50.0	50.0	57.8	57.4	900-923 bp	1.0	73.9
ndhF-rpl32_p4	GGAACGGGAAGTGGAATGAA	AAAGCGGTCCAATATCCCTTC	50.0	47.6	57.8	58.1	905-928 bp	1.0	70.5
ndhF-rpl32_p5	CGGGAAGTGGAATGAAAGGT	AAAGCGGTCCAATATCCCTTC	50.0	47.6	57.8	58.1	901-924 bp	1.0	70.5