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psbE-petL

LSC · 63614-64823 · 1210 bp

Back to Gentianopsis

Pi 0.00331

MarkerSeek score 60.90

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1210
variable_sites: 6
pi: 0.00331
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACGAATAACCAACCCGCAAT	ATCGTTAAAGCCGCGAGTAG	45.0	50.0	58.2	58.2	1373-1380 bp	1.0	77.7
psbE-petL_p2	TGCTGACGAATAACCAACCC	ATCGTTAAAGCCGCGAGTAG	50.0	50.0	58.2	58.2	1378-1385 bp	1.0	77.6
psbE-petL_p3	CGAATAACCAACCCGCAATG	ATCGTTAAAGCCGCGAGTAG	50.0	50.0	57.8	58.2	1372-1379 bp	1.0	77.5
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ATCGTTAAAGCCGCGAGTAG	55.0	50.0	58.2	58.2	1282-1289 bp	1.0	77.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	TTATCGTTAAAGCCGCGAGT	45.0	45.0	58.2	57.7	1375-1382 bp	1.0	76.7