Hotspot

ycf4-cemA

LSC · 59477-60016 · 540 bp

Back to Gentianopsis

Pi 0.00495

MarkerSeek score 39.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 4
pi: 0.00495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCATTCCCCTAACGCGTATT	ACCAGGGGTGGGAATACTTT	50.0	50.0	57.8	58.2	676-689 bp	1.0	76.7
ycf4-cemA_p2	CCATTCCCCTAACGCGTATT	AATGAGAGAGAAAGCCCCCA	50.0	50.0	57.8	58.3	724-737 bp	1.0	75.9
ycf4-cemA_p3	CCATTCCCCTAACGCGTATT	CCAGGGGTGGGAATACTTTC	50.0	55.0	57.8	57.6	675-688 bp	1.0	75.1
ycf4-cemA_p4	CCCCTAACGCGTATTGATGA	ACCAGGGGTGGGAATACTTT	50.0	50.0	57.5	58.2	671-684 bp	1.0	74.6
ycf4-cemA_p5	TCCCCTAACGCGTATTGATG	ACCAGGGGTGGGAATACTTT	50.0	50.0	57.5	58.2	672-685 bp	1.0	74.6