Hotspot

ndhC-trnV-UAC

LSC · 49381-50463 · 1083 bp

Back to Gentianopsis

Pi 0.0138

MarkerSeek score 66.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1083
variable_sites: 20
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1177-1214 bp	1.0	82.7
ndhC-trnV-UAC_p2	AATTGGGGCTAAAACTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.2	57.8	1181-1218 bp	1.0	82.5
ndhC-trnV-UAC_p3	GGTCCTTTCCTAATTGGGGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.2	57.8	1192-1229 bp	1.0	82.0
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1176-1213 bp	1.0	79.9
ndhC-trnV-UAC_p5	AATTGGGGCTAAAACTCCGG	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.2	57.5	1180-1217 bp	1.0	79.8