Hotspot

trnT-GGU-psbD

LSC · 30459-31725 · 1267 bp

Back to Gentianopsis

Pi 0.00211

MarkerSeek score 56.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1267
variable_sites: 4
pi: 0.00211
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.3	58.0	1386-1412 bp	1.0	78.0
trnT-GGU-psbD_p2	CCCCTTTAACTCAGCGGTAG	GAAACGGTCCCTACGTAACC	55.0	55.0	57.7	58.0	1422-1448 bp	1.0	77.8
trnT-GGU-psbD_p3	TAAGCCCCTTTAACTCAGCG	GAAACGGTCCCTACGTAACC	50.0	55.0	57.6	58.0	1426-1452 bp	1.0	77.1
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	GACCAGCCTACAAACACGAA	50.0	50.0	58.3	58.1	1403-1429 bp	1.0	77.0
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	AAACACGAAACGGTCCCTAC	50.0	50.0	58.3	58.1	1392-1418 bp	1.0	77.0