Hotspot

psbM-trnD-GUC

LSC · 28323-29177 · 855 bp

Back to Gentianopsis

Pi 0.0039
MarkerSeek score 47.30
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
855
variable_sites
5
pi
0.0039
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AATGCCCGCAAAACTACTCA CACCTATTCAATTCCCGGGG 45.0 55.0 58.1 58.0 1559-1574 bp 1.0 79.5
psbM-trnD-GUC_p2 ATGCCCGCAAAACTACTCAA CACCTATTCAATTCCCGGGG 45.0 55.0 58.1 58.0 1558-1573 bp 1.0 79.5
psbM-trnD-GUC_p3 TGCCCGCAAAACTACTCAAT CACCTATTCAATTCCCGGGG 45.0 55.0 58.1 58.0 1557-1572 bp 1.0 79.5
psbM-trnD-GUC_p4 AAAAATCGGCGAGGCTTACT CACCTATTCAATTCCCGGGG 45.0 55.0 57.9 58.0 1606-1621 bp 1.0 79.4
psbM-trnD-GUC_p5 TGCCCGCAAAACTACTCAAT ACCTATTCAATTCCCGGGGA 45.0 50.0 58.1 58.1 1556-1571 bp 1.0 78.9