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atpH-atpI

LSC · 11794-12887 · 1094 bp

Back to Gentianopsis

Pi 0.00427

MarkerSeek score 61.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 7
pi: 0.00427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AAGCAAGTCCTACAGCCAAC	GCTGCGGCTTATATAGGTGA	50.0	50.0	58.1	57.3	1196-1197 bp	1.0	73.8
atpH-atpI_p2	AAGCAAGTCCTACAGCCAAC	ATTTTTGCAACCCTAGCTGC	50.0	45.0	58.1	57.3	1211-1212 bp	1.0	73.7
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	GCTGCGGCTTATATAGGTGA	55.0	50.0	58.1	57.3	1250-1251 bp	1.0	73.5
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	GCTGCGGCTTATATAGGTGA	55.0	50.0	57.9	57.3	1254-1255 bp	1.0	73.4
atpH-atpI_p5	TACCCTCTACAGCTTGACCC	ATTTTTGCAACCCTAGCTGC	55.0	45.0	58.1	57.3	1265-1266 bp	1.0	73.4