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psbK-psbI

LSC · 6061-6429 · 369 bp

Back to Gentianopsis

Pi 0.00542

MarkerSeek score 46.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 3
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	TGGAAAATTGCCTGAAGCCT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.0	57.4	510-573 bp	1.0	59.6
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.1	57.4	427-490 bp	1.0	59.4
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACGAAGAGTTTGAGAGTAAGCA	50.0	40.9	57.9	57.4	422-485 bp	1.0	59.4
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	419-482 bp	1.0	57.4
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	ACGAAGAGTTTGAGAGTAAGCA	45.0	40.9	58.3	57.4	420-483 bp	1.0	57.4