Hotspot

rpl32-trnL-UAG

SSC · 110252-111230 · 979 bp

Back to Gentiana

Pi 0.0754

MarkerSeek score 75.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 979
variable_sites: 362
pi: 0.0754
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGGATATTGGACCGCT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.2	57.9	650-1119 bp	0.232558	58.8
rpl32-trnL-UAG_p2	AGAAGGGATATTGGACCGCT	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.2	57.8	643-1112 bp	0.232558	58.3
rpl32-trnL-UAG_p3	AGAAGGGATATTGGACCGCT	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.2	57.8	648-1117 bp	0.232558	58.2
rpl32-trnL-UAG_p4	AGAAGGGATATTGGACCGCT	AGTCCTGACATCGAAAAGCC	50.0	50.0	58.2	57.9	1580-1607 bp	0.093023	50.9
rpl32-trnL-UAG_p5	AGAAGGGATATTGGACCGCT	GTCCTGACATCGAAAAGCCT	50.0	50.0	58.2	57.9	1579-1606 bp	0.093023	50.9