Hotspot

rpl20-rps12

LSC · 67202-68006 · 805 bp

Back to Gentiana

Pi 0.0279

MarkerSeek score 71.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 150
pi: 0.0279
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAATGGGCCCC	AATGTCACTAAATCCCCCGC	55.0	50.0	58.2	57.9	879-971 bp	1.0	89.7
rpl20-rps12_p2	GTAAGTCTTGAATGGGCCCC	CTCAACGACGAGGAACATGT	55.0	50.0	58.2	57.9	845-937 bp	1.0	89.6
rpl20-rps12_p3	GTAAGTCTTGAATGGGCCCC	GCTCTTAGGGGATGTCCTCA	55.0	55.0	58.2	58.2	861-953 bp	1.0	88.5
rpl20-rps12_p4	TAAGTCTTGAATGGGCCCCT	AATGTCACTAAATCCCCCGC	50.0	50.0	58.3	57.9	878-970 bp	0.953488	87.2
rpl20-rps12_p5	TAAGTCTTGAATGGGCCCCT	CTCAACGACGAGGAACATGT	50.0	50.0	58.3	57.9	844-936 bp	0.953488	87.1