Hotspot

petA-psbJ

LSC · 61399-62245 · 847 bp

Back to Gentiana

Pi 0.0513

MarkerSeek score 76.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 214
pi: 0.0513
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGGATTTGGTCAGGGAGATG	ACGGTCTAGCTGTACCTACC	55.0	55.0	58.0	58.0	1168-1484 bp	1.0	91.3
petA-psbJ_p2	CGGATTTGGTCAGGGAGATG	GGTCTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1166-1482 bp	1.0	91.3
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	TCCCTATTCATTGCGGGTTG	55.0	50.0	58.0	57.9	1397-1713 bp	1.0	91.3
petA-psbJ_p4	GCCCCTACCGATCGTATTTC	ACGGTCTAGCTGTACCTACC	55.0	55.0	57.9	58.0	1617-1933 bp	1.0	91.0
petA-psbJ_p5	GCCCCTACCGATCGTATTTC	GGTCTAGCTGTACCTACCGT	55.0	55.0	57.9	58.0	1615-1931 bp	1.0	91.0