Hotspot

trnT-UGU-trnL-UAA

LSC · 45424-46166 · 743 bp

Back to Gentiana

Pi 0.0511

MarkerSeek score 72.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 191
pi: 0.0511
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCCGTTCTGTAAGGCCATAA	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	981-1339 bp	0.860465	86.1
trnT-UGU-trnL-UAA_p2	GCCGTTCTGTAAGGCCATAA	CAAAAGGGTCGGGGAAGAAA	50.0	50.0	58.0	58.0	1668-1821 bp	0.837209	85.1
trnT-UGU-trnL-UAA_p3	CCTGACAGAGTTGGAAGCTC	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	644-994 bp	0.790698	82.9
trnT-UGU-trnL-UAA_p4	GCCGTTCTGTAAGGCCATAA	TATAGAATAGGGCGGGTGCA	50.0	50.0	58.0	58.0	1735-1879 bp	0.511628	70.8
trnT-UGU-trnL-UAA_p5	GCCGTTCTGTAAGGCCATAA	GTTGATCTACAGGCTGTGGG	50.0	55.0	58.0	58.0	1632 bp	0.023256	40.4