Hotspot

trnC-GCA-petN

LSC · 26641-27539 · 899 bp

Back to Gentiana

Pi 0.0421

MarkerSeek score 75.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 232
pi: 0.0421
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	334-976 bp	1.0	91.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	328-970 bp	1.0	89.8
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	352-994 bp	0.953488	87.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCCACTTCTTCCCCACACTA	50.0	50.0	58.1	57.6	778-1026 bp	0.953488	86.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	346-988 bp	0.953488	85.8