Hotspot

trnS-GCU-trnS-CGA

LSC · 6799-7354 · 556 bp

Back to Gentiana

Pi 0.0522

MarkerSeek score 80.50

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 556
variable_sites: 161
pi: 0.0522
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	CAATCCGTCGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	503-659 bp	1.0	73.1
trnS-GCU-trnS-CGA_p2	AATCCGTCGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	502-658 bp	1.0	73.1
trnS-GCU-trnS-CGA_p3	AGCAATCCGTCGCTTTAGTC	ACCACTAAACTATACCCGCT	50.0	45.0	58.4	55.6	505-661 bp	1.0	70.3
trnS-GCU-trnS-CGA_p4	CGTCGCTTTAGTCCACTCAG	ACCACTAAACTATACCCGCT	55.0	45.0	58.4	55.6	498-654 bp	1.0	70.1
trnS-GCU-trnS-CGA_p5	GCTTTAGTCCACTCAGCCAT	ACCACTAAACTATACCCGCT	50.0	45.0	57.6	55.6	494-650 bp	1.0	69.9