Hotspot

trnQ-UUG-psbK

LSC · 5692-6058 · 367 bp

Back to Gentiana

Pi 0.0501

MarkerSeek score 71.70

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 90
pi: 0.0501
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	CAGATCCCTATCAAACTACAA	55.0	38.1	57.6	52.4	437-502 bp	0.906977	48.3
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	CAGATCCCTATCAAACTACAAA	55.0	36.4	57.6	53.3	437-502 bp	0.906977	48.3
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	CAGATCCCTATCAAACTACAA	50.0	38.1	58.7	52.4	395-460 bp	0.906977	48.3
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	CAGATCCCTATCAAACTACAAA	50.0	36.4	58.7	53.3	395-460 bp	0.906977	48.3
trnQ-UUG-psbK_p5	GAATAGCGGGACCAAAACCC	CAGATCCCTATCAAACTACAA	55.0	38.1	58.9	52.4	404-469 bp	0.906977	48.3