primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 CTGCCTTAATCCACTTGGCT TTCGGCGGTTCCTTATTCAG 20 20 50.0 50.0 57.9 58.0 0.0 7.2 0.153331 76 439 657-875 1.000000 90.8 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CTGCCTTAATCCACTTGGCT TCAGTGCTATGCATGGTTCC 20 20 50.0 50.0 57.9 58.0 0.0 10.2 0.177475 76 439 641-859 1.000000 90.6 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 ACTGCCTTAATCCACTTGGC TTCGGCGGTTCCTTATTCAG 20 20 50.0 50.0 58.2 58.0 0.0 7.2 0.179118 76 439 658-876 1.000000 90.6 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 CTGCCTTAATCCACTTGGCT CAGTGCTATGCATGGTTCCT 20 20 50.0 50.0 57.9 58.0 0.0 10.2 0.183233 76 439 640-858 1.000000 90.5 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 CTGCCTTAATCCACTTGGCT TGCTGCTTGGCCTGTAATAG 20 20 50.0 50.0 57.9 58.0 0.0 3.3 0.177475 76 439 448-666 0.651163 76.6 trnK-UUU_p1 trnK-UUU 1 CCAAGCACACGCATTCTCTA GCAGTTTGTTTGTTAGTTCAAGG 20 23 50.0 39.1 58.3 57.1 0.0 0.0 4.223592 1717 4277 2850-2901 0.720930 45.1 trnK-UUU_p2 trnK-UUU 2 AAGCACACGCATTCTCTACA GCAGTTTGTTTGTTAGTTCAAGG 20 23 45.0 39.1 57.5 57.1 0.0 0.0 4.404906 1717 4277 2848-2899 0.720930 43.6 trnK-UUU_p3 trnK-UUU 3 AGCACACGCATTCTCTACAA GCAGTTTGTTTGTTAGTTCAAGG 20 23 45.0 39.1 57.5 57.1 0.0 0.0 4.404906 1717 4277 2847-2898 0.720930 43.6 trnK-UUU_p4 trnK-UUU 4 TACAGAAGCGACCCCATAGG GCAGTTTGTTTGTTAGTTCAAGG 20 23 55.0 39.1 58.6 57.1 0.0 0.0 4.539228 1717 4277 2943-2994 0.720930 42.5 trnK-UUU_p5 trnK-UUU 5 ATCCAGTTACAGAAGCGACC GCAGTTTGTTTGTTAGTTCAAGG 20 23 50.0 39.1 57.3 57.1 0.0 0.0 4.624028 1717 4277 2950-3000 0.697674 40.8 trnK-UUU-trnQ-UUG_p1 trnK-UUU-trnQ-UUG 1 CCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 18 20 55.6 55.0 55.7 58.1 0.0 16.9 4.378925 4278 5619 478-1495 1.000000 57.0 trnK-UUU-trnQ-UUG_p2 trnK-UUU-trnQ-UUG 2 CCGTTGAGTTAGCAACCC TTCGGAGGTTCGAATCCTTC 18 20 55.6 50.0 55.7 57.3 0.0 16.9 4.970297 4278 5619 482-1499 1.000000 52.2 trnK-UUU-trnQ-UUG_p3 trnK-UUU-trnQ-UUG 3 CCGTTGAGTTAGCAACCC TAAGGCAACGGGTTTTGGTC 18 20 55.6 50.0 55.7 58.7 0.0 0.0 4.982317 4278 5619 508-1525 1.000000 52.1 trnK-UUU-trnQ-UUG_p4 trnK-UUU-trnQ-UUG 4 CCGTTGAGTTAGCAACCC GGGTTTTGGTCCCGCTATTC 18 20 55.6 55.0 55.7 58.9 0.0 0.0 5.202314 4278 5619 499-1516 1.000000 52.0 trnK-UUU-trnQ-UUG_p5 trnK-UUU-trnQ-UUG 5 CCGTTGAGTTAGCAACCC AAATCTAAATGGGGCGTGGC 18 20 55.6 50.0 55.7 58.9 0.0 0.0 5.191547 4278 5619 535-1552 0.976744 51.1 trnQ-UUG-psbK_p1 trnQ-UUG-psbK 1 GATATGCTCTGGGACGGAAG CAGATCCCTATCAAACTACAA 20 21 55.0 38.1 57.6 52.4 0.0 0.0 7.083770 5692 6058 437-502 0.906977 48.3 trnQ-UUG-psbK_p2 trnQ-UUG-psbK 2 GATATGCTCTGGGACGGAAG CAGATCCCTATCAAACTACAAA 20 22 55.0 36.4 57.6 53.3 0.0 0.0 7.175989 5692 6058 437-502 0.906977 48.3 trnQ-UUG-psbK_p3 trnQ-UUG-psbK 3 GACCAAAACCCGTTGCCTTA CAGATCCCTATCAAACTACAA 20 21 50.0 38.1 58.7 52.4 0.0 0.0 7.316019 5692 6058 395-460 0.906977 48.3 trnQ-UUG-psbK_p4 trnQ-UUG-psbK 4 GACCAAAACCCGTTGCCTTA CAGATCCCTATCAAACTACAAA 20 22 50.0 36.4 58.7 53.3 0.0 0.0 7.408238 5692 6058 395-460 0.906977 48.3 trnQ-UUG-psbK_p5 trnQ-UUG-psbK 5 GAATAGCGGGACCAAAACCC CAGATCCCTATCAAACTACAA 20 21 55.0 38.1 58.9 52.4 0.0 0.0 7.536017 5692 6058 404-469 0.906977 48.3 trnS-GCU-trnS-CGA_p1 trnS-GCU-trnS-CGA 1 CAATCCGTCGCTTTAGTCCA ACCACTAAACTATACCCGCT 20 20 50.0 45.0 58.0 55.6 0.0 0.0 2.358738 6799 7354 503-659 1.000000 73.1 trnS-GCU-trnS-CGA_p2 trnS-GCU-trnS-CGA 2 AATCCGTCGCTTTAGTCCAC ACCACTAAACTATACCCGCT 20 20 50.0 45.0 58.0 55.6 0.0 0.0 2.360126 6799 7354 502-658 1.000000 73.1 trnS-GCU-trnS-CGA_p3 trnS-GCU-trnS-CGA 3 AGCAATCCGTCGCTTTAGTC ACCACTAAACTATACCCGCT 20 20 50.0 45.0 58.4 55.6 0.0 0.0 2.712578 6799 7354 505-661 1.000000 70.3 trnS-GCU-trnS-CGA_p4 trnS-GCU-trnS-CGA 4 CGTCGCTTTAGTCCACTCAG ACCACTAAACTATACCCGCT 20 20 55.0 45.0 58.4 55.6 0.0 0.0 2.735295 6799 7354 498-654 1.000000 70.1 trnS-GCU-trnS-CGA_p5 trnS-GCU-trnS-CGA 5 GCTTTAGTCCACTCAGCCAT ACCACTAAACTATACCCGCT 20 20 50.0 45.0 57.6 55.6 0.9 0.0 2.764104 6799 7354 494-650 1.000000 69.9 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CCTCATTTCCATCAACGAGC CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 56.9 58.1 0.0 0.0 1.204692 25324 26569 874-1360 0.930233 79.6 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 ACTCCCTCATTTCCATCAACG CCGGATTTGAACTGGGGAAA 21 20 47.6 50.0 58.0 58.1 0.0 0.0 1.090878 25324 26569 878-1364 0.883721 78.6 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 TCCCTCATTTCCATCAACGAG CCGGATTTGAACTGGGGAAA 21 20 47.6 50.0 57.7 58.1 0.0 0.0 1.348638 25324 26569 876-1362 0.930233 78.4 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 ACTCCCTCATTTCCATCAACG AAAAGGATTTGCAGTCCCCC 21 20 47.6 50.0 58.0 58.4 0.0 14.6 1.364241 25324 26569 860-1346 0.906977 77.4 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 CTCCCTCATTTCCATCAACGA CCGGATTTGAACTGGGGAAA 21 20 47.6 50.0 57.7 58.1 0.0 0.0 1.348638 25324 26569 877-1363 0.883721 76.6 trnC-GCA-petN_p1 trnC-GCA-petN 1 TTTCCCCAGTTCAAATCCGG TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.088172 26641 27539 334-976 1.000000 91.3 trnC-GCA-petN_p2 trnC-GCA-petN 2 TTTCCCCAGTTCAAATCCGG CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.1 57.8 0.0 0.0 0.273256 26641 27539 328-970 1.000000 89.8 trnC-GCA-petN_p3 trnC-GCA-petN 3 GGGGGACTGCAAATCCTTTT TTAAAGCAGCCCAAGCAAGA 20 20 50.0 45.0 58.4 58.0 14.7 0.0 0.361535 26641 27539 352-994 0.953488 87.2 trnC-GCA-petN_p4 trnC-GCA-petN 4 TTTCCCCAGTTCAAATCCGG TCCACTTCTTCCCCACACTA 20 20 50.0 50.0 58.1 57.6 0.0 0.0 0.491192 26641 27539 778-1026 0.953488 86.2 trnC-GCA-petN_p5 trnC-GCA-petN 5 GGGGGACTGCAAATCCTTTT CAGCCCAAGCAAGACTTACT 20 20 50.0 50.0 58.4 57.8 14.7 0.0 0.546619 26641 27539 346-988 0.953488 85.8 trnE-UUC-trnT-GGU_p1 trnE-UUC-trnT-GGU 1 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 20 20 55.0 50.0 58.3 58.0 0.0 0.0 0.269359 29762 30257 438-646 1.000000 89.8 trnE-UUC-trnT-GGU_p2 trnE-UUC-trnT-GGU 2 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 20 20 50.0 50.0 57.7 58.0 0.0 0.0 0.287509 29762 30257 417-625 1.000000 89.7 trnE-UUC-trnT-GGU_p3 trnE-UUC-trnT-GGU 3 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 20 20 55.0 55.0 58.3 58.1 0.0 0.0 0.327456 29762 30257 454-625 0.930233 86.6 trnE-UUC-trnT-GGU_p4 trnE-UUC-trnT-GGU 4 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 20 20 50.0 55.0 57.7 58.1 0.0 0.0 0.345606 29762 30257 433-604 0.930233 86.4 trnE-UUC-trnT-GGU_p5 trnE-UUC-trnT-GGU 5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 20 20 55.0 50.0 58.3 58.1 0.0 0.0 0.349611 29762 30257 451-622 0.930233 86.4 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 20 20 50.0 40.0 58.3 53.0 0.0 6.7 5.273069 30332 31542 903-1278 1.000000 52.0 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GTGGTAGAGTAACGCCATGG TTTACCAAGGGCTATAGTCA 20 20 55.0 40.0 58.1 53.0 7.6 6.7 5.054330 30332 31542 926-1301 0.976744 51.1 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 TCAGTGGTAGAGTAACGCCA TTTACCAAGGGCTATAGTCA 20 20 50.0 40.0 58.1 53.0 0.0 6.7 5.076485 30332 31542 929-1304 0.976744 51.1 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 GTGGTAGAGTAACGCCATGG TTACCAAGGGCTATAGTCAT 20 20 55.0 40.0 58.1 52.8 7.6 6.7 5.305690 30332 31542 925-1300 0.976744 51.1 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 TCAGTGGTAGAGTAACGCCA TTACCAAGGGCTATAGTCAT 20 20 50.0 40.0 58.1 52.8 0.0 6.7 5.327845 30332 31542 928-1303 0.976744 51.1 psaA-ycf3_p1 psaA-ycf3 1 TGTGAGTTGGATCGCTTAGC ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.051103 40594 41443 1180-1279 0.604651 73.4 psaA-ycf3_p2 psaA-ycf3 2 ATGTATTTGATGTCCCGCCC ATCAAGCCGCTGAGTATTGG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.059702 40594 41443 1506-1605 0.604651 72.4 psaA-ycf3_p3 psaA-ycf3 3 CTTCTGGTTCCGGCGAAC ACGAGGCGTTTCGAATAA 18 18 61.1 44.4 58.4 54.4 9.3 15.7 8.074425 40594 41443 789-915 1.000000 52.0 psaA-ycf3_p4 psaA-ycf3 4 ACTTCTGGTTCCGGCGAA ACGAGGCGTTTCGAATAA 18 18 55.6 44.4 58.9 54.4 9.3 15.7 8.492775 40594 41443 790-916 1.000000 52.0 psaA-ycf3_p5 psaA-ycf3 5 ACTTCTGGTTCCGGCGAAC ACGAGGCGTTTCGAATAA 19 18 57.9 44.4 60.3 54.4 9.3 15.7 8.938539 40594 41443 790-916 1.000000 52.0 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCCGTTCTGTAAGGCCATAA ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 58.0 58.0 5.0 0.0 0.037304 45424 46166 981-1339 0.860465 86.1 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GCCGTTCTGTAAGGCCATAA CAAAAGGGTCGGGGAAGAAA 20 20 50.0 50.0 58.0 58.0 5.0 0.0 0.042974 45424 46166 1668-1821 0.837209 85.1 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 CCTGACAGAGTTGGAAGCTC ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 3.3 0.0 0.096476 45424 46166 644-994 0.790698 82.9 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GCCGTTCTGTAAGGCCATAA TATAGAATAGGGCGGGTGCA 20 20 50.0 50.0 58.0 58.0 5.0 0.0 0.051036 45424 46166 1735-1879 0.511628 70.8 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCCGTTCTGTAAGGCCATAA GTTGATCTACAGGCTGTGGG 20 20 50.0 55.0 58.0 58.0 5.0 29.7 0.062519 45424 46166 1632-1632 0.023256 40.4 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 TCAAGTCCCTCTATCCCCAC TCTACCAGCTGAGCTATCCC 20 20 55.0 55.0 57.8 58.0 0.0 17.4 0.178753 46617 46957 385-385 0.023256 39.5 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 TCAAGTCCCTCTATCCCCAC TTTGAACTGGTGACACGAGG 20 20 55.0 50.0 57.8 58.1 0.0 20.1 0.228143 46617 46957 421-421 0.023256 39.1 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 TCAAGTCCCTCTATCCCCAC GTGCCAGGAACCAGATTTGA 20 20 55.0 50.0 57.8 58.1 0.0 0.0 0.266548 46617 46957 436-436 0.023256 38.8 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 TCAAGTCCCTCTATCCCCAC TCAGTCCTCTGCTCTACCAG 20 20 55.0 55.0 57.8 57.9 0.0 0.0 0.312546 46617 46957 397-397 0.023256 38.4 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 TCAAGTCCCTCTATCCCCAC CATGTGCCAGGAACCAGATT 20 20 55.0 50.0 57.8 58.2 0.0 0.0 0.332879 46617 46957 439-439 0.023256 38.3 psaI-ycf4_p1 psaI-ycf4 1 ATTTCCGGCAATTGCAATGG AGACCCCGCGATAAGTTCTA 20 20 45.0 50.0 58.0 57.9 30.3 8.2 0.089974 57986 58402 462-544 0.976744 90.3 psaI-ycf4_p2 psaI-ycf4 2 ATTTCCGGCAATTGCAATGG TAGACCCCGCGATAAGTTCT 20 20 45.0 50.0 58.0 57.9 30.3 8.2 0.089974 57986 58402 463-545 0.976744 90.3 psaI-ycf4_p3 psaI-ycf4 3 ATTTCCGGCAATTGCAATGG TCTAGACCCCGCGATAAGTT 20 20 45.0 50.0 58.0 57.9 30.3 8.2 0.089974 57986 58402 465-547 0.976744 90.3 psaI-ycf4_p4 psaI-ycf4 4 ATTTCCGGCAATTGCAATGG TTCTAGACCCCGCGATAAGT 20 20 45.0 50.0 58.0 57.9 30.3 8.2 0.089974 57986 58402 466-548 0.976744 90.3 psaI-ycf4_p5 psaI-ycf4 5 GGCAATTGCAATGGCTTCTT AGACCCCGCGATAAGTTCTA 20 20 45.0 50.0 57.9 57.9 32.4 8.2 0.166508 57986 58402 456-538 0.976744 89.7 petA-psbJ_p1 petA-psbJ 1 CGGATTTGGTCAGGGAGATG ACGGTCTAGCTGTACCTACC 20 20 55.0 55.0 58.0 58.0 0.0 15.1 0.090847 61399 62245 1168-1484 1.000000 91.3 petA-psbJ_p2 petA-psbJ 2 CGGATTTGGTCAGGGAGATG GGTCTAGCTGTACCTACCGT 20 20 55.0 55.0 58.0 58.0 0.0 11.1 0.090847 61399 62245 1166-1482 1.000000 91.3 petA-psbJ_p3 petA-psbJ 3 CGGATTTGGTCAGGGAGATG TCCCTATTCATTGCGGGTTG 20 20 55.0 50.0 58.0 57.9 0.0 0.0 0.092293 61399 62245 1397-1713 1.000000 91.3 petA-psbJ_p4 petA-psbJ 4 GCCCCTACCGATCGTATTTC ACGGTCTAGCTGTACCTACC 20 20 55.0 55.0 57.9 58.0 15.1 15.1 0.125867 61399 62245 1617-1933 1.000000 91.0 petA-psbJ_p5 petA-psbJ 5 GCCCCTACCGATCGTATTTC GGTCTAGCTGTACCTACCGT 20 20 55.0 55.0 57.9 58.0 15.1 11.1 0.125867 61399 62245 1615-1931 1.000000 91.0 rpl20-rps12_p1 rpl20-rps12 1 GTAAGTCTTGAATGGGCCCC AATGTCACTAAATCCCCCGC 20 20 55.0 50.0 58.2 57.9 22.3 0.0 0.288814 67202 68006 879-971 1.000000 89.7 rpl20-rps12_p2 rpl20-rps12 2 GTAAGTCTTGAATGGGCCCC CTCAACGACGAGGAACATGT 20 20 55.0 50.0 58.2 57.9 22.3 0.0 0.302025 67202 68006 845-937 1.000000 89.6 rpl20-rps12_p3 rpl20-rps12 3 GTAAGTCTTGAATGGGCCCC GCTCTTAGGGGATGTCCTCA 20 20 55.0 55.0 58.2 58.2 22.3 1.1 0.441318 67202 68006 861-953 1.000000 88.5 rpl20-rps12_p4 rpl20-rps12 4 TAAGTCTTGAATGGGCCCCT AATGTCACTAAATCCCCCGC 20 20 50.0 50.0 58.3 57.9 22.3 0.0 0.371554 67202 68006 878-970 0.953488 87.2 rpl20-rps12_p5 rpl20-rps12 5 TAAGTCTTGAATGGGCCCCT CTCAACGACGAGGAACATGT 20 20 50.0 50.0 58.3 57.9 22.3 0.0 0.384764 67202 68006 844-936 0.953488 87.1 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AGAAGGGATATTGGACCGCT TCCTAAGAGCAGCGTGTCTA 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.316463 110252 111230 650-1119 0.232558 58.8 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AGAAGGGATATTGGACCGCT AGCAGCGTGTCTACCAATTT 20 20 50.0 45.0 58.2 57.8 0.0 0.0 0.377190 110252 111230 643-1112 0.232558 58.3 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AGAAGGGATATTGGACCGCT CTAAGAGCAGCGTGTCTACC 20 20 50.0 55.0 58.2 57.8 0.0 0.0 0.388481 110252 111230 648-1117 0.232558 58.2 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AGAAGGGATATTGGACCGCT AGTCCTGACATCGAAAAGCC 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.292791 110252 111230 1580-1607 0.093023 50.9 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AGAAGGGATATTGGACCGCT GTCCTGACATCGAAAAGCCT 20 20 50.0 50.0 58.2 57.9 0.0 0.0 0.292791 110252 111230 1579-1606 0.093023 50.9 ycf1_p1 ycf1 1 CATCGCCCCATCTCAATTGA TTGGACGGGAATGAATGAGG 20 20 50.0 50.0 58.0 57.6 8.1 0.0 0.446887 120935 121054 308-443 0.906977 84.7 ycf1_p2 ycf1 2 CATCGCCCCATCTCAATTGA TGGACGGGAATGAATGAGGA 20 20 50.0 50.0 58.0 58.4 8.1 0.0 0.449622 120935 121054 307-442 0.906977 84.7 ycf1_p3 ycf1 3 CATCGCCCCATCTCAATTGA AAGTGTCCCATCTCAACCCT 20 20 50.0 50.0 58.0 58.3 8.1 0.0 0.281075 120935 121054 280-415 0.581395 73.0 ycf1_p4 ycf1 4 GTTGATTCAAAGCATCGCCC AAGTGTCCCATCTCAACCCT 20 20 50.0 50.0 58.1 58.3 0.0 0.0 0.339239 120935 121054 421-427 0.209302 53.7 ycf1_p5 ycf1 5 TGACTACAGTTATTCGCGGC AAGTGTCCCATCTCAACCCT 20 20 50.0 50.0 58.1 58.3 6.1 0.0 0.335283 120935 121054 848-848 0.023256 38.2