Hotspot

ycf1

SSC · 110226-115412 · 5187 bp

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Pi 0.0851
MarkerSeek score 78.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5187
variable_sites
1189
pi
0.0851
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CATCCCCGTTTGATATGCCA TCCGAGCTCAGGTTATGGAA 50.0 50.0 58.0 58.1 1008-1050 bp 0.714286 75.3
ycf1_p2 CATCCCCGTTTGATATGCCA AAGGTATCAGCAACAACCGG 50.0 50.0 58.0 58.2 973-1015 bp 0.714286 75.1
ycf1_p3 CATCCCCGTTTGATATGCCA AATCCAGTCGGTCATTGTGG 50.0 50.0 58.0 57.9 1088-1130 bp 0.714286 74.9
ycf1_p4 GACCCCTTCTTCCACGTTTT TCCGAGCTCAGGTTATGGAA 50.0 50.0 58.0 58.1 641-668 bp 0.714286 74.8
ycf1_p5 GACCCCTTCTTCCACGTTTT AATCCAGTCGGTCATTGTGG 50.0 50.0 58.0 57.9 721-748 bp 0.714286 74.2