Hotspot

rpl16

LSC · 77851-79126 · 1276 bp

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Pi 0.065

MarkerSeek score 83.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1276
variable_sites: 221
pi: 0.065
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	TCCGCTACAAACCATTCGAG	50.0	50.0	58.0	58.0	1674-1716 bp	0.857143	84.8
rpl16_p2	AACGGGTCTGTGGTTGAATC	TCCGCTACAAACCATTCGAG	50.0	50.0	58.1	58.0	1633-1675 bp	0.857143	84.3
rpl16_p3	CCCCAACCCCAAAAAGATCA	TCCGCTACAAACCATTCGAG	50.0	50.0	58.0	58.0	2017-2059 bp	0.571429	68.5
rpl16_p4	ACCCCAACCCCAAAAAGATC	TCCGCTACAAACCATTCGAG	50.0	50.0	58.0	58.0	2018-2060 bp	0.571429	68.5
rpl16_p5	CCCCAACCCCAAAAAGATCA	CTTGCCGAATTTTTAGCCGG	50.0	50.0	58.0	58.1	2190-2232 bp	0.571429	67.4